Recombinant Mouse Siglec-3/CD33 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10102-SL-050
Recombinant Mouse Siglec-3/CD33 Fc Chimera Protein, CF SDS-PAGE
1 Image
Product Details
FAQs
Reviews

Recombinant Mouse Siglec-3/CD33 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Siglec‑H Fc Chimera (Catalog # 10264-SH) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Siglec‑3 Fc Chimera (Catalog # 10102-SL) that produces 50% of the optimal binding response is 5-20 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Siglec-3/CD33 protein
Mouse Siglec-3/CD33
(Gln17-Glu240)
Accession # Q63994
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Gln17 inferred from enymatic pyroglutamate treatment revealing Asp18
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
52 kDa
SDS-PAGE
55-75 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10102-SL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Siglec-3/CD33 Fc Chimera (Catalog # 10102-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-75 kDa and 110-150 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Siglec-3/CD33

Siglec-3 (sialic acid binding Ig-like lectin 3), also known as myeloid cell surface antigen CD33 and GP67, is an I-type (Ig-type) lectin belonging to the Ig superfamily. Siglecs are characterized by an N-terminal Ig-like V-type domain, which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Fourteen human and nine mouse Siglecs have been characterized and are divided into 2 families: CD33 related and evolutionarily conserved (3). Mature mouse Siglec-3 consists of a 224 amino acid (aa) extracellular domain (ECD), containing one IgV and one IgC2 domain and shares 56% aa identity with human Siglec-3. Mouse Siglec-3 is expressed on myeloid precursors in the bone marrow, mostly in the mature stages of the granulocytic lineage (4). Each Siglec has a distinct preference for binding the various types of sialylated glycans found on the surface of mammalian cells and they most likely evolved to regulate host immune responses via the recognition of self-glycans (5). Unlike human Siglec-3, mouse Siglec-3 lacks a canonical cytoplasmic ITIM domain. Instead, mouse Siglec-3 possesses a charged amino acid in its transmembrane domain, which may interact with an ITAM adaptor protein (6). Additionally, mouse Siglec-3 binds sialic acids found on mucins rather than alpha 2-3- or alpha 2-6-linked sialic acids on lactosamine units to which human Siglec-3 binds (7, 8). These differences suggest mouse and human Siglec-3 might not be functionally identical (8). Human Siglec-3 continues to be a therapeutic target for the treatment of acute myeloid leukemia and is a high potential risk factor for Alzheimer's (9).

Long Name
Sialic Acid Binding Ig-like Lectin 3
Entrez Gene IDs
945 (Human); 12489 (Mouse)
Alternate Names
CD33 antigen (gp67); CD33 antigen; CD33 molecule; CD33; FLJ00391; gp67; myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec3; Siglec-3; SIGLEC3gp67

Manufacturing Specifications

  1. Crocker, P.R. and Varki, A. (2001) Trends Immunol. 22:337.
  2. Crocker, P.R. and Varki, A. (2001) Immunology 103:137.
  3. Macauley, M.S. et al. (2014) Nat. Rev. Immunol. 14:653.
  4. Varki, A. et al. (2017) Essentials of Glycobiology. Chapter 35.
  5. Paulson, J. et al. (2012) Ann. N. Y. Acad. Sci. 1253:37.
  6. Pillai, S. et al. (2012) Ann. Rev. Immunol. 30:357.
  7. Brinkman-Van der Linden, E.C. and Varki, A. (2000) J. Bio. Chem. 275:8633.
  8. Brinkman-Van der Linden, E.C. et al. (2003) Mol. Cell. Bio. 23:4199.
  9. Malik, M. et al. (2013) J. Neurosci. 33:13320.

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Mouse Siglec-3/CD33 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Siglec-3/CD33 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Mouse Siglec-3/CD33 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review