Recombinant Mouse Skint2/B7S3 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10279-B7-050
Recombinant Mouse Skint2/B7S3 Fc Chimera Protein, CF Bioactivity
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Recombinant Mouse Skint2/B7S3 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.5-5.0 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Skint2/B7S3 protein
Mouse Skint2/B7S3
(Ser22-Lys239)
Accession # NP_001272892.1
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ser22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
52 kDa
SDS-PAGE
48-64 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10279-B7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Mouse Skint2/B7S3 Fc Chimera (Catalog # 10279-B7) inhibits IL-2 secretion by mouse T cells in the presence of Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841). The ED50 for this effect is 0.5-5.0 μg/mL.

SDS-PAGE SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse Skint2/B7S3 Fc Chimera (Catalog # 10279-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 48-64 kDa and 95-130 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Skint2/B7S3

B7S3 (B7 superfamily member 3) is a member of the B7 superfamily which play a major role in maintaining the appropriate threshold of T cell activation. B7S3 has two isoforms expressed in both lymphoid and non-lymphoid tissues (1). The mature extracellular domain (ECD) of mouse B7S3 contains an IgV-like domain, and a half IgC-like domain (1). Within the ECD, mouse B7S3 shares 89% amino acid sequence identity with rat B7S3. A putative receptor for B7S3 is constitutively expressed on APCs and induced on T cells upon activation. Mouse B7S3 mFc chimera inhibited CD4 and CD8 T cell proliferation and IL-2 production, as well as effector cytokine production. Interestingly, the B7S3 genes in mouse and human have distinct structural features (1).

References
  1. Yang, Y. et al. (2007) J. Immunol. 178:3661.
Long Name
Selection and Upkeep of Intraepithelial T Cells 2
Entrez Gene IDs
329919 (Mouse)
Alternate Names
B7S3; Skint2

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