Recombinant Mouse Skint2/B7S3 Fc Chimera Protein, CF Summary
Accession # NP_001272892.1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse Skint2/B7S3 Fc Chimera (Catalog # 10279-B7) inhibits IL-2 secretion by mouse T cells in the presence of Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841). The ED50 for this effect is 0.5-5.0 µg/mL.
1 μg/lane of Recombinant Mouse Skint2/B7S3 Fc Chimera (Catalog # 10279-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 48-64 kDa and 95-130 kDa, respectively.
B7S3 (B7 superfamily member 3) is a member of the B7 superfamily which play a major role in maintaining the appropriate threshold of T cell activation. B7S3 has two isoforms expressed in both lymphoid and non-lymphoid tissues (1). The mature extracellular domain (ECD) of mouse B7S3 contains an IgV-like domain, and a half IgC-like domain (1). Within the ECD, mouse B7S3 shares 89% amino acid sequence identity with rat B7S3. A putative receptor for B7S3 is constitutively expressed on APCs and induced on T cells upon activation. Mouse B7S3 mFc chimera inhibited CD4 and CD8 T cell proliferation and IL-2 production, as well as effector cytokine production. Interestingly, the B7S3 genes in mouse and human have distinct structural features (1).
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