Recombinant Mouse SR-AI/MSR1 Protein, CF

Catalog # Availability Size / Price Qty
1797-MS-050
Product Details
Citations (1)
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Recombinant Mouse SR-AI/MSR1 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse SR‑AI/MSR at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA) with a linear range of 2-100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse SR-AI/MSR protein
Trp79-Ser454, with an N-terminal 9-His tag
Accession #
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
42 kDa
SDS-PAGE
60-70 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1797-MS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: SR-AI/MSR

The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share the common ability to bind and internalize modified forms of Low Density Lipoproteins (mLDL) (1 - 3). Family members are classified alphabetically. The A class include four proteins: the three subtypes of SR-A (AI, AII, and AIII) that are generated by alternative splicing of the same gene, and a structurally similar protein named MARCO (4). All A class SRs are multidomain trimeric type II membrane proteins. SR-AI has an N-terminal cytoplasmic domain, a transmembrane domain, a spacer domain, an alpha -helical coiled coil, a collagen-like domain and a C-terminal cysteine-rich domain. SR-A is expressed by most tissue macrophages, dendritic cells and Kupffer cells. It is also highly expressed by microglia in neonatal as well as Alzheimer’ Disease brains. SR-AI binds a broad range of polyanionic ligands including modified proteins (e.g. Oxidized, acetylated or maleylated LDL, Advanced glycation end-product proteins), polyribonucleotides (polyguanosine and polyinosine), polysaccharides (dextran sulfate, fucoidan), phospholipids (phosphatidylserine), bacterial products (lipopolysaccharide and lipoteichoic acid) and selected chemical compounds (silica, crocidolite asbestos). The ligand-binding region has been localized to a positively charged region in the carboxyl end of the collagen-like domain. Based on its ligand binding characteristics, SR-AI is implicated in many physiological and pathophysiological functions. Studies using SR-A knockout mouse have also suggested roles of SR-A in atherogenesis, host defense and innate immunity, acquired immune responses, macrophage adhesion, and phagocytosis of apoptotic cells (1 - 3).

References
  1. Daugherty, A. et al. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs 2:223.
  2. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
  3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
  4. Elomaa, O. et al. (1995) Cell 80:603.
Long Name
Macrophage Scavenger Receptor Types I and II
Entrez Gene IDs
4481 (Human); 20288 (Mouse); 25073 (Rat)
Alternate Names
CD204 antigen; CD204; Macrophage acetylated LDL receptor I and II; macrophage scavenger receptor 1; macrophage scavenger receptor type III; MSR1; phSR1; phSR2; SCARA1; SCARA1macrophage scavenger receptor types I and II; Scavenger receptor class A member 1; scavenger receptor class A, member 1; SR-A; SRAI; SR-AI

Citation for Recombinant Mouse SR-AI/MSR1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Interferon-induced mechanosensing defects impede apoptotic cell clearance in lupus.
    Authors: Li H, Fu Y, Wu Q, Zhou Y, Crossman D, Yang P, Li J, Luo B, Morel L, Kabarowski J, Yagita H, Ware C, Hsu H, Mountz J
    J Clin Invest, 2015;125(7):2877-90.
    Species: Mouse
    Sample Types: Serum
    Applications: Bioassay

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