Recombinant Mouse SR-BI Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9644-SR-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse SR-BI Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834. The ED50 for this effect is 0.4-2 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived mouse SR-BI protein
Mouse SR-BI
(Pro33-Tyr443)
Accession # Q61009-1
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Pro33
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
74 kDa
SDS-PAGE
85-120 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9644-SR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SR-BI

Scavenger Receptor, class B, member 1 (SR-BI), gene name SCARB1, is also known as CD36L1 (CD36-like 1) or CLA-1 (CD36 and LIMPII analogous 1) (1-5). SR-BI is a transmembrane glycoprotein found on macrophages, liver cells and other steroidogenic cells as a lipoprotein receptor. The 509 amino acid (aa) mouse SR-BI contains a central extracellular domain (ECD), flanked by N- and C-terminal transmembrane domains. A mouse splice variant differs at the C-terminal cytoplasmic domain (SR-BII, 506 aa) (2). The mouse SR-BI ECD shares 81% and 92% aa sequence identity with human and rat SR-BI, respectively. SR-BI functions in reverse cholesterol transport (RCT), which is thought to be anti-atherogenic by facilitating transport of cholesteryl esters from macrophages back to the liver for degradation (3). In rodent hepatocytes, SR-BI is the main receptor mediating RCT, while human hepatocytes also express a second mediator, CETP (cholesteryl ester transfer protein) (3-5). On endothelial cells, signaling through SR-BI activates nitric oxide production, which attenuates monocyte adhesion (6). On adrenocortical cells, SR-BI mediates uptake of cholesteryl esters from HDL for the synthesis of glucocorticoid hormones such as cortisol (3-5). On platelets, HDL binding to surface SR-BI inhibits aggregation and increases platelet survival time (3-5). SR-BI and its SR-BII isoform also bind bacterial lipopolysaccharides, facilitating uptake of various bacteria by cells such as peritoneal macrophages (7, 8). This uptake enhances inflammatory responses which, unless properly controlled, can result in sepsis (8-10).

References
  1. Calvo, D. and M. A. Vega (1993) J. Biol. Chem. 268:18929.
  2. Webb, N.R. et al. (1998) J. Biol. Chem. 273:15241.
  3. Chadwick, A.C. and D. Sahoo (2013) Curr. Opin. Endocrinol. Diabetes Obes. 20:124.
  4. Hoekstra, M. et al. (2012) Curr. Opin. Lipidol. 23:127.
  5. Vergeer, M. et al. (2011) N. Engl. J. Med. 364:136.
  6. Guo, L. et al. (2011) J. Lipid Res. 52:2272.
  7. Vishnyakova, T.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16888.
  8. Baranova, I.N. et al. (2012) J. Immunol. 188:1371.
  9. Leelahavanichkul, A. et al. (2012) J. Immunol. 188:2749.
  10. Guo, L. et al. (2009) J. Biol. Chem. 284:19826.    
Long Name
Scavenger Receptor Class B, Member I
Entrez Gene IDs
949 (Human); 20778 (Mouse); 25073 (Rat)
Alternate Names
CD36 and LIMPII analogous 1; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1; CD36 antigen; CD36L1; CD36L1scavenger receptor class B type 1; CLA1 CD36 antigen-like 1; CLA-1 HDLQTL6; CLA1; Collagen type I receptor, thrombospondin receptor-like 1; HDLQTL6; MGC138242; SCARB1; scavenger receptor class B type III; scavenger receptor class B, member 1; SRB1 scavenger receptor class B member 1; SR-B1; SRBI; SR-BI

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