Recombinant Mouse Thrombopoietin R Fc Chimera Protein, CF

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Recombinant Mouse Thrombopoietin R Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit the Tpo-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.1-0.4 µg/mL in the presence of 4 ng/mL of Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO).
Mouse myeloma cell line, NS0-derived mouse Thrombopoietin R/Tpo R protein
Met Mouse Tpo R
Accession # CAA52031
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
79.6 kDa (monomer)
95-110 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
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Background: Thrombopoietin R/Tpo R

Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-mpl), is a type I membrane protein that is a member of the hematopoietin/cytokine receptor superfamily. Thrombopoietin (Tpo) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. Receptor dimerization occurs upon ligand binding. Downstream signaling cascades utilize the Ras/MAP and JAK/STAT pathways (1). In humans, variations at the 3’ end on the molecule result in the formation of 3 distinct mRNA species, a P-form, a K-form, and a potentially secreted form. The predominant form in humans is the P-form, which encodes a full length receptor. In mouse, mRNA for a full length form, a potentially secreted form, and a form lacking 8 amino acids in the extra-cytoplasmic domain has been detected (2). The human and mouse receptors share approximately 81% amino acid identity. Tpo R is expressed at low levels in various cell types including hematopoietic progenitor cells, megakaryocytes and platelets.

  1. Kuter, D.J. and J. Li (2001) in Cytokine Reference, p. 1953. Oppenhiem, J.J. and M. Feldmann eds. Academic Press.
  2. Kaushansky, K. and J.G. Drachman (2002) Oncogene 21:3359.
Long Name
Thrombopoietin Receptor
Entrez Gene IDs
4352 (Human); 17480 (Mouse)
Alternate Names
CD110 antigen; CD110; c-Mpl; MPL; MPLV; Myeloproliferative leukemia protein; myeloproliferative leukemia virus oncogene; Proto-oncogene c-Mpl; Thrombopoietin R; thrombopoietin receptor; TpoR; TPO-R; TPORMPLV

Citation for Recombinant Mouse Thrombopoietin R Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Megakaryocytes are essential for HSC quiescence through the production of thrombopoietin.
    Authors: Nakamura-Ishizu, Ayako, Takubo, Keiyo, Fujioka, Masato, Suda, Toshio
    Biochem Biophys Res Commun, 2014;454(2):353-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization


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