Recombinant Mouse Thrombopoietin Protein

(26 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.4-2.4 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Ser22-Thr356
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser22
  • Predicted Molecular Mass
    35 kDa
  • SDS-PAGE
    80-90 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
488-TO
 
488-TO/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO) stimulates cell proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.4-2.4 ng/mL.
1 μg/lane of Recombinant Mouse Thrombopoietin/Tpo was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 80-90 kDa.
Background: Thrombopoietin/Tpo
Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1-3). The 356 amino acid (aa) mouse Tpo precursor is cleaved to yield the 335 aa mature protein. Mature mouse Tpo shares 71% and 81% aa sequence homology with human and rat Tpo, respectively. It is an 80-85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of mouse Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10-13). It is cleaved by platelet-derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling (15, 16).
  • References:
    1. Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
    2. Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
    3. Li, J. et al. (1999) Br. J. Haematol. 106:345.
    4. Bartley, T.D. et al. (1994) Cell 77:1117.
    5. de Sauvage, F.J. et al. (1994) Nature 369:533.
    6. Marcucci, R. and M. Romano  (2008) Biochim. Biophys. Acta 1782:427.
    7. Kaushansky, K. et al. (1994) Nature 369:568.
    8. Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.
    9. Broudy, V.C. et al. (1995) Blood 85:1719.
    10. Lok, S.I. et al. (1994) Nature 369:565.
    11. Chen, J. et al. (1995) Blood 86:4054.
    12. Oda, A. et al. (1996) Blood 87:4664.
    13. Van Os, E. et al. (2003) Br. J. Haematol. 121:482.
    14. Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.
    15. Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.
    16. Samoylenko, A. et al. (2008) Cell. Signal. 20:154.
  • Entrez Gene IDs:
    7066 (Human); 21832 (Mouse); 81811 (Rat)
  • Alternate Names:
    Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MK-CSF; ML; MPL ligand; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34(+) Cells for Correction of Fabry Disease
    Authors: J Huang, A Khan, BC Au, DL Barber, L López-Vásq, NL Prokopishy, M Boutin, M Rothe, JW Rip, M Abaoui, MS Nagree, S Dworski, A Schambach, A Keating, ML West, J Klassen, PV Turner, S Sirrs, CA Rupar, C Auray-Blai, R Foley, JA Medin
    Mol Ther Methods Clin Dev, 2017;5(0):241-258.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Glucocorticoid hormone-induced chromatin remodeling enhances human hematopoietic stem cell homing and engraftment
    Authors: B Guo, X Huang, S Cooper, HE Broxmeyer
    Nat. Med, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Protected graft copolymer-formulated fibroblast growth factors mitigate the lethality of partial body irradiation injury
    Authors: GM Castillo, A Nishimoto-, CC Jones, KK Kabirov, A Zakharov, AV Lyubimov
    PLoS ONE, 2017;12(2):e0171703.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Standard
  4. Modulation of splicing catalysis for therapeutic targeting of leukemia with mutations in genes encoding spliceosomal proteins
    Nat. Med., 2016;22(6):672-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors
    Nat Commun, 2016;7(0):13396.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis.
    Authors: Wang F, Gao J, Malisani A, Xi X, Han W, Wan X
    BMC Biotechnol, 2015;15(0):105.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Flow-induced protein kinase A-CREB pathway acts via BMP signaling to promote HSC emergence.
    Authors: Kim P, Nakano H, Das P, Chen M, Rowe R, Chou S, Ross S, Sakamoto K, Zon L, Schlaeger T, Orkin S, Nakano A, Daley G
    J Exp Med, 2015;212(5):633-48.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  8. Angptl4 is upregulated under inflammatory conditions in the bone marrow of mice, expands myeloid progenitors, and accelerates reconstitution of platelets after myelosuppressive therapy.
    Authors: Schumacher A, Denecke B, Braunschweig T, Stahlschmidt J, Ziegler S, Brandenburg L, Stope M, Martincuks A, Vogt M, Gortz D, Camporeale A, Poli V, Muller-Newen G, Brummendorf T, Ziegler P
    J Hematol Oncol, 2015;8(0):64.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. CD41 marks the initial myelo-erythroid lineage specification in adult mouse hematopoiesis: redefinition of murine common myeloid progenitor.
    Authors: Miyawaki K, Arinobu Y, Iwasaki H, Kohno K, Tsuzuki H, Iino T, Shima T, Kikushige Y, Takenaka K, Miyamoto T, Akashi K
    Stem Cells, 2015;33(3):976-87.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  10. FlnA binding to PACSIN2 F-BAR domain regulates membrane tubulation in megakaryocytes and platelets.
    Authors: Begonja A, Pluthero F, Suphamungmee W, Giannini S, Christensen H, Leung R, Lo R, Nakamura F, Lehman W, Plomann M, Hoffmeister K, Kahr W, Hartwig J, Falet H
    Blood, 2015;126(1):80-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Dnmt3a loss predisposes murine hematopoietic stem cells to malignant transformation.
    Authors: Mayle A, Yang L, Rodriguez B, Zhou T, Chang E, Curry C, Challen G, Li W, Wheeler D, Rebel V, Goodell M
    Blood, 2015;125(4):629-38.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  12. Dynamin 2-dependent endocytosis is required for normal megakaryocyte development in mice.
    Authors: Bender M, Giannini S, Grozovsky R, Jonsson T, Christensen H, Pluthero F, Ko A, Mullally A, Kahr W, Hoffmeister K, Falet H
    Blood, 2015;125(6):1014-24.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. Copy number variation is a fundamental aspect of the placental genome.
    Authors: Hannibal R, Chuong E, Rivera-Mulia J, Gilbert D, Valouev A, Baker J
    PLoS Genet, 2014;10(5):e1004290.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  14. The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function.
    Authors: Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H
    Nature, 2014;514(7520):98-101.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Computational promoter modeling identifies the modes of transcriptional regulation in hematopoietic stem cells.
    Authors: Park S, Umemoto T, Saito-Adachi M, Shiratsuchi Y, Yamato M, Nakai K
    PLoS ONE, 2014;9(4):e93853.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  16. Sox17-mediated maintenance of fetal intra-aortic hematopoietic cell clusters.
    Authors: Nobuhisa I, Osawa M, Uemura M, Kishikawa Y, Anani M, Harada K, Takagi H, Saito K, Kanai-Azuma M, Kanai Y, Iwama A, Taga T
    Mol Cell Biol, 2014;34(11):1976-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  17. Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets.
    Authors: Weston W, Zayas J, Perez R, George J, Jurecic R
    Sci Rep, 2014;4(0):5199.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. Lis1 regulates asymmetric division in hematopoietic stem cells and in leukemia.
    Authors: Zimdahl, Bryan, Ito, Takahiro, Blevins, Allen, Bajaj, Jeevisha, Konuma, Takaaki, Weeks, Joi, Koechlein, Claire S, Kwon, Hyog You, Arami, Omead, Rizzieri, David, Broome, H Elizab, Chuah, Charles, Oehler, Vivian G, Sasik, Roman, Hardiman, Gary, Reya, Tannisht
    Nat Genet, 2014;46(3):245-52.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  19. Hes1 promotes blast crisis in chronic myelogenous leukemia through MMP-9 upregulation in leukemic cells.
    Authors: Nakahara F, Kitaura J, Uchida T, Nishida C, Togami K, Inoue D, Matsukawa T, Kagiyama Y, Enomoto Y, Kawabata K, Chen-Yi L, Komeno Y, Izawa K, Oki T, Nagae G, Harada Y, Harada H, Otsu M, Aburatani H, Heissig B, Hattori K, Kitamura T
    Blood, 2014;123(25):3932-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  20. Identification of transcription factors for lineage-specific ESC differentiation.
    Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
    Stem Cell Reports, 2013;1(6):545-59.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  21. Induction of a hemogenic program in mouse fibroblasts.
    Authors: Pereira C, Chang B, Qiu J, Niu X, Papatsenko D, Hendry C, Clark N, Nomura-Kitabayashi A, Kovacic J, Ma'ayan A, Schaniel C, Lemischka I, Moore K
    Cell Stem Cell, 2013;13(2):205-18.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  22. Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment.
    Authors: Cook BD, Liu S, Evans T
    Blood, 2011;117(24):6489-97.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  23. Insulin-like growth factor-binding protein 2 secreted by a tumorigenic cell line supports ex vivo expansion of mouse hematopoietic stem cells.
    Authors: Huynh H, Iizuka S, Kaba M, Kirak O, Zheng J, Lodish HF, Zhang CC
    Stem Cells, 2008;26(6):1628-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. The lineage-c-Kit+Sca-1+ cell response to Escherichia coli bacteremia in Balb/c mice.
    Authors: Zhang P, Nelson S, Bagby GJ, Siggins R, Shellito JE, Welsh DA
    Stem Cells, 2008;26(7):1778-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  25. SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity.
    Authors: Yilmaz OH, Kiel MJ, Morrison SJ
    Blood, 2005;107(3):924-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  26. Combinations of cytokines promote survival of mice and limit acute radiation damage in concert with amelioration of vascular damage.
    Authors: Van der Meeren A, Mouthon MA, Vandamme M, Squiban C, Aigueperse J
    Radiat. Res., 2004;161(5):549-59.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
Expand to show all 26 Citations

Have you used Recombinant Mouse Thrombopoietin Protein?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

  • Formulations

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information