Recombinant Mouse Thrombopoietin Protein

Formulations:
Catalog # Availability Size / Price Qty
488-TO-005
488-TO-025
488-TO-200
Recombinant Mouse Thrombopoietin Protein Data
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Product Details
Citations (37)
FAQs
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Recombinant Mouse Thrombopoietin Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.4-2.4 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Thrombopoietin/Tpo protein
Ser22-Thr356
Accession #
N-terminal Sequence
Analysis
Ser22
Predicted Molecular Mass
35 kDa
SDS-PAGE
80-90 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

488-TO

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

488-TO/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO) stimulates cell proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50for this effect is 0.4-2.4 ng/mL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse Thrombopoietin/Tpo was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 80-90 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: Thrombopoietin/Tpo

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1-3). The 356 amino acid (aa) mouse Tpo precursor is cleaved to yield the 335 aa mature protein. Mature mouse Tpo shares 71% and 81% aa sequence homology with human and rat Tpo, respectively. It is an 80-85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of mouse Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10-13). It is cleaved by platelet-derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling (15, 16).

References
  1. Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
  2. Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
  3. Li, J. et al. (1999) Br. J. Haematol. 106:345.
  4. Bartley, T.D. et al. (1994) Cell 77:1117.
  5. de Sauvage, F.J. et al. (1994) Nature 369:533.
  6. Marcucci, R. and M. Romano  (2008) Biochim. Biophys. Acta 1782:427.
  7. Kaushansky, K. et al. (1994) Nature 369:568.
  8. Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.
  9. Broudy, V.C. et al. (1995) Blood 85:1719.
  10. Lok, S.I. et al. (1994) Nature 369:565.
  11. Chen, J. et al. (1995) Blood 86:4054.
  12. Oda, A. et al. (1996) Blood 87:4664.
  13. Van Os, E. et al. (2003) Br. J. Haematol. 121:482.
  14. Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.
  15. Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.
  16. Samoylenko, A. et al. (2008) Cell. Signal. 20:154.
Entrez Gene IDs
7066 (Human); 21832 (Mouse); 81811 (Rat)
Alternate Names
Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MKCSF; MK-CSF; ML; MPL ligand; MPLLG; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THCYT1; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF

Citations for Recombinant Mouse Thrombopoietin Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Hto, Tritiated Amino Acid Exposure and External Exposure Induce Differential Effects on Hematopoiesis and Iron Metabolism
    Authors: JM Bertho, D Kereselidz, L Manens, C Culeux, V Magneron, J Surette, M Blimkie, L Bertrand, H Wyatt, M Souidi, I Dublineau, N Priest, JR Jourdain
    Sci Rep, 2019;9(1):19919.
    Species: Mouse
    Sample Types: Plasma
    Applications: Multiplex Standard
  2. SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells
    Authors: CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi
    Cell Rep, 2019;28(13):3329-3337.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling
    Authors: ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer
    J. Clin. Invest., 2019;130(0):2555-2570.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Mutation-specific signaling profiles and kinase inhibitor sensitivities of juvenile myelomonocytic leukemia revealed by induced pluripotent stem cells
    Authors: SK Tasian, JA Casas, D Posocco, S Gandre-Bab, AL Gagne, G Liang, ML Loh, MJ Weiss, DL French, ST Chou
    Leukemia, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Mapping Active Gene-Regulatory Regions in Human Repopulating Long-Term HSCs
    Authors: P Wünsche, ESP Eckert, T Holland-Le, A Paruzynski, A Hotz-Wagen, R Fronza, T Rath, I Gil-Farina, M Schmidt, C von Kalle, C Klein, CR Ball, F Herbst, H Glimm
    Cell Stem Cell, 2018;23(1):132-146.e9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Wild-type Kras expands and exhausts hematopoietic stem cells
    Authors: JP Sasine, HA Himburg, CM Termini, M Roos, E Tran, L Zhao, J Kan, M Li, Y Zhang, SC de Barros, DS Rao, CM Counter, JP Chute
    JCI Insight, 2018;3(11):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Wild-type Kras expands and exhausts hematopoietic stem cells
    Authors: JP Sasine, HA Himburg, CM Termini, M Roos, E Tran, L Zhao, J Kan, M Li, Y Zhang, SC de Barros, DS Rao, CM Counter, JP Chute
    JCI Insight, 2018;3(11):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Global H3.3 dynamic deposition defines its bimodal role in cell fate transition
    Authors: HT Fang, CA El Farran, QR Xing, LF Zhang, H Li, B Lim, YH Loh
    Nat Commun, 2018;9(1):1537.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. ETV2/ER71 regulates hematopoietic regeneration by promoting hematopoietic stem cell proliferation
    Authors: CX Xu, TJ Lee, N Sakurai, K Krchma, F Liu, D Li, T Wang, K Choi
    J. Exp. Med., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34(+) Cells for Correction of Fabry Disease
    Authors: J Huang, A Khan, BC Au, DL Barber, L López-Vásq, NL Prokopishy, M Boutin, M Rothe, JW Rip, M Abaoui, MS Nagree, S Dworski, A Schambach, A Keating, ML West, J Klassen, PV Turner, S Sirrs, CA Rupar, C Auray-Blai, R Foley, JA Medin
    Mol Ther Methods Clin Dev, 2017;5(0):241-258.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. ?-Arrestin2 mediates progression of murine primary myelofibrosis
    Authors: LA Rein, JW Wisler, J Kim, B Theriot, L Huang, T Price, H Yang, M Chen, W Chen, D Sipkins, Y Fedoriw, JK Walker, RT Premont, RJ Lefkowitz
    JCI Insight, 2017;2(24):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. ZSCAN10 expression corrects the genomic instability of iPSCs from aged donors
    Authors: M Skamagki, C Correia, P Yeung, T Baslan, S Beck, C Zhang, CA Ross, L Dang, Z Liu, S Giunta, TP Chang, J Wang, A Ananthanar, M Bohndorf, B Bosbach, J Adjaye, H Funabiki, J Kim, S Lowe, JJ Collins, CW Lu, H Li, R Zhao, K Kim
    Nat. Cell Biol., 2017;19(9):1037-1048.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Differentiation
  13. Protected graft copolymer-formulated fibroblast growth factors mitigate the lethality of partial body irradiation injury
    Authors: GM Castillo, A Nishimoto-, CC Jones, KK Kabirov, A Zakharov, AV Lyubimov
    PLoS ONE, 2017;12(2):e0171703.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA (Standard)
  14. Glucocorticoid hormone-induced chromatin remodeling enhances human hematopoietic stem cell homing and engraftment
    Authors: B Guo, X Huang, S Cooper, HE Broxmeyer
    Nat. Med, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Modulation of splicing catalysis for therapeutic targeting of leukemia with mutations in genes encoding spliceosomal proteins
    Nat. Med., 2016;22(6):672-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors
    Nat Commun, 2016;7(0):13396.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Dynamin 2-dependent endocytosis is required for normal megakaryocyte development in mice.
    Authors: Bender M, Giannini S, Grozovsky R, Jonsson T, Christensen H, Pluthero F, Ko A, Mullally A, Kahr W, Hoffmeister K, Falet H
    Blood, 2015;125(6):1014-24.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Dnmt3a loss predisposes murine hematopoietic stem cells to malignant transformation.
    Authors: Mayle A, Yang L, Rodriguez B, Zhou T, Chang E, Curry C, Challen G, Li W, Wheeler D, Rebel V, Goodell M
    Blood, 2015;125(4):629-38.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. FlnA binding to PACSIN2 F-BAR domain regulates membrane tubulation in megakaryocytes and platelets.
    Authors: Begonja A, Pluthero F, Suphamungmee W, Giannini S, Christensen H, Leung R, Lo R, Nakamura F, Lehman W, Plomann M, Hoffmeister K, Kahr W, Hartwig J, Falet H
    Blood, 2015;126(1):80-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. CD41 marks the initial myelo-erythroid lineage specification in adult mouse hematopoiesis: redefinition of murine common myeloid progenitor.
    Authors: Miyawaki K, Arinobu Y, Iwasaki H, Kohno K, Tsuzuki H, Iino T, Shima T, Kikushige Y, Takenaka K, Miyamoto T, Akashi K
    Stem Cells, 2015;33(3):976-87.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Angptl4 is upregulated under inflammatory conditions in the bone marrow of mice, expands myeloid progenitors, and accelerates reconstitution of platelets after myelosuppressive therapy.
    Authors: Schumacher A, Denecke B, Braunschweig T, Stahlschmidt J, Ziegler S, Brandenburg L, Stope M, Martincuks A, Vogt M, Gortz D, Camporeale A, Poli V, Muller-Newen G, Brummendorf T, Ziegler P
    J Hematol Oncol, 2015;8(0):64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Flow-induced protein kinase A-CREB pathway acts via BMP signaling to promote HSC emergence.
    Authors: Kim P, Nakano H, Das P, Chen M, Rowe R, Chou S, Ross S, Sakamoto K, Zon L, Schlaeger T, Orkin S, Nakano A, Daley G
    J Exp Med, 2015;212(5):633-48.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis.
    Authors: Wang F, Gao J, Malisani A, Xi X, Han W, Wan X
    BMC Biotechnol, 2015;15(0):105.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Sox17-mediated maintenance of fetal intra-aortic hematopoietic cell clusters.
    Authors: Nobuhisa I, Osawa M, Uemura M, Kishikawa Y, Anani M, Harada K, Takagi H, Saito K, Kanai-Azuma M, Kanai Y, Iwama A, Taga T
    Mol Cell Biol, 2014;34(11):1976-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Computational promoter modeling identifies the modes of transcriptional regulation in hematopoietic stem cells.
    Authors: Park S, Umemoto T, Saito-Adachi M, Shiratsuchi Y, Yamato M, Nakai K
    PLoS ONE, 2014;9(4):e93853.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Lis1 regulates asymmetric division in hematopoietic stem cells and in leukemia.
    Authors: Zimdahl, Bryan, Ito, Takahiro, Blevins, Allen, Bajaj, Jeevisha, Konuma, Takaaki, Weeks, Joi, Koechlein, Claire S, Kwon, Hyog You, Arami, Omead, Rizzieri, David, Broome, H Elizab, Chuah, Charles, Oehler, Vivian G, Sasik, Roman, Hardiman, Gary, Reya, Tannisht
    Nat Genet, 2014;46(3):245-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets.
    Authors: Weston W, Zayas J, Perez R, George J, Jurecic R
    Sci Rep, 2014;4(0):5199.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Hes1 promotes blast crisis in chronic myelogenous leukemia through MMP-9 upregulation in leukemic cells.
    Authors: Nakahara F, Kitaura J, Uchida T, Nishida C, Togami K, Inoue D, Matsukawa T, Kagiyama Y, Enomoto Y, Kawabata K, Chen-Yi L, Komeno Y, Izawa K, Oki T, Nagae G, Harada Y, Harada H, Otsu M, Aburatani H, Heissig B, Hattori K, Kitamura T
    Blood, 2014;123(25):3932-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Copy number variation is a fundamental aspect of the placental genome.
    Authors: Hannibal R, Chuong E, Rivera-Mulia J, Gilbert D, Valouev A, Baker J
    PLoS Genet, 2014;10(5):e1004290.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function.
    Authors: Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H
    Nature, 2014;514(7520):98-101.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Induction of a hemogenic program in mouse fibroblasts.
    Authors: Pereira C, Chang B, Qiu J, Niu X, Papatsenko D, Hendry C, Clark N, Nomura-Kitabayashi A, Kovacic J, Ma'ayan A, Schaniel C, Lemischka I, Moore K
    Cell Stem Cell, 2013;13(2):205-18.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Identification of transcription factors for lineage-specific ESC differentiation.
    Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
    Stem Cell Reports, 2013;1(6):545-59.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment.
    Authors: Cook BD, Liu S, Evans T
    Blood, 2011;117(24):6489-97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  34. The lineage-c-Kit+Sca-1+ cell response to Escherichia coli bacteremia in Balb/c mice.
    Authors: Zhang P, Nelson S, Bagby GJ, Siggins R, Shellito JE, Welsh DA
    Stem Cells, 2008;26(7):1778-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Insulin-like growth factor-binding protein 2 secreted by a tumorigenic cell line supports ex vivo expansion of mouse hematopoietic stem cells.
    Authors: Huynh H, Iizuka S, Kaba M, Kirak O, Zheng J, Lodish HF, Zhang CC
    Stem Cells, 2008;26(6):1628-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity.
    Authors: Yilmaz OH, Kiel MJ, Morrison SJ
    Blood, 2005;107(3):924-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Combinations of cytokines promote survival of mice and limit acute radiation damage in concert with amelioration of vascular damage.
    Authors: Van der Meeren A, Mouthon MA, Vandamme M, Squiban C, Aigueperse J
    Radiat. Res., 2004;161(5):549-59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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