Recombinant Mouse TLR3 Protein, CF

R&D Systems | Catalog # 3005-TR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse TLR3 Protein (3005-TR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q99MB1

Applications

Bioactivity
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Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TLR3 protein
Cys29-Leu705, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys29

Predicted Molecular Mass

77.7 kDa

SDS-PAGE

114-127 kDa, reducing conditions

Activity

Measured by its ability to inhibit poly I:C induced IL-8 secretion by HEK293 human embryonic kidney cells transfected with TLR3.
The ED50 for this effect is 2-12 µg/mL in the presence of 0.2 µg/mL of poly I:C.

Formulation, Preparation, and Storage

3005-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TLR3

Mouse TLR3 is a 116 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules (1, 2). There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (3). The mouse TLR3 cDNA encodes a 905 amino acid (aa) precursor that contains a 25 aa signal sequence, a 680 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 179 aa cytoplasmic region (4). The horseshoe shaped ECD (5, 6) contains 23 leucine-rich repeats, and the cytoplasmic domain contains one
Toll/IL-1 receptor (TIR) domain. The ECD of mouse TLR3 shares 90%, 79%, and 77% aa sequence identity with the ECD of rat, human, and bovine TLR3, respectively. TLR3 is found in phagosomes (7), where the acidic pH enables binding of internalized double stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells (8-11). Ligand binding by TLR3 induces receptor dimerization (5, 6, 8), leading to the release of inflammatory cytokines and dendritic cell maturation (9, 11-13). Mouse TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes (13-15), and is upregulated by IFN-beta and LPS (9, 14). TLR3 expression is also induced on lung fibroblasts and epithelial cells by respiratory syncytial virus infection (12).

References

  1. Sen, G.C. and S.N. Sarkar (2005) Cytokine Growth Factor Rev. 16:1.
  2. Schroder, M. and A.G. Bowie (2005) Trends Immunol. 26:462.
  3. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  4. Applequist, S.E. et al. (2002) Int. Immunol. 14:1065.
  5. Choe, J. et al. (2005) Science 309:581.
  6. Bell, J.K. et al. (2005) Proc. Natl. Acad. Sci. USA 102:10976.
  7. Nishiya, T. et al. (2002) J. Biol. Chem. 280:37107.
  8. de Bouteiller, O. et al. (2005) J. Biol. Chem. 280:38133.
  9. Alexopoulou, L. et al. (2001) Nature 413:732.
  10. Aksoy, E. et al. (2005) J. Biol. Chem. 280:277.
  11. Kariko, K. et al. (2004) J. Biol. Chem. 279:12542.
  12. Rudd, B.D. et al. (2005) J. Virol. 79:3350.
  13. Farina, C. et al. (2005) J. Neuroimmunol. 159:12.
  14. Heinz, S. et al. (2003) J. Biol. Chem. 278:21502.
  15. Wang, T. et al. (2004) Nat. Med. 10:1366.

Long Name

Toll-like Receptor 3

Alternate Names

CD283

Entrez Gene IDs

7098 (Human); 142980 (Mouse); 364594 (Rat)

Gene Symbol

TLR3

UniProt

Q99MB1

Additional TLR3 Products

Product Documents for Recombinant Mouse TLR3 Protein, CF

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Product Specific Notices for Recombinant Mouse TLR3 Protein, CF

For research use only

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