hh Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein, CF 462-TR-010/CF: R&D Systems

Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein, CF

(43 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 5‑15 ng/mL in the presence of 2.5 µg/mL of a
    cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
    An E. coli-expressed Recombinant Mouse (rm) TRANCE (aa 158-317) (Catalog # 462-TEC) is also available. The ED50 for the E. coli-expressed rmTRANCE is 0.5‑2 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Arg72-Asp316, with an N-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    His
  • Predicted Molecular Mass
    28 kDa
  • SDS-PAGE
    36 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
462-TR/CF
 
462-TR
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 3 months from date of receipt, 2 to 8 °C as supplied.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Data Images

Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR/CF) induces osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cell line. The ED50 for this effect is 5-15 ng/mL in the presence of 2.5 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050)

1 μg/lane of Recombinant Mouse TRANCE/TNFSF11/RANK L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 36 kDa.
Background: TRANCE/TNFSF11/RANK L

TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokines, osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. In the TNF superfamily nomenclature, TRANCE is referred to as TNFSF11. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The murine TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

  • References:
    1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
    2. Anderson, D.M. et al. (1997) Nature 390:175.
    3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
    4. Kong, Y-Y. et al. (1999) Nature 397:315.
  • Long Name:
    TNF-related Activation-induced Cytokine
  • Entrez Gene IDs:
    8600 (Human); 21943 (Mouse); 117516 (Rat)
  • Alternate Names:
    CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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Applications
Sample Type
  1. CCL11, a novel mediator of inflammatory bone resorption
    Authors: E Kindstedt, CK Holm, R Sulniute, I Martinez-C, R Lundmark, P Lundberg
    Sci Rep, 2017;7(1):5334.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Irisin prevents and restores bone loss and muscle atrophy in hind-limb suspended mice
    Authors: G Colaianni, T Mongelli, C Cuscito, P Pignataro, L Lippo, G Spiro, A Notarnicol, I Severi, G Passeri, G Mori, G Brunetti, B Moretti, U Tarantino, SC Colucci, JE Reseland, R Vettor, S Cinti, M Grano
    Sci Rep, 2017;7(1):2811.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Zoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB
    Authors: SY Gao, GS Zheng, L Wang, YJ Liang, SE Zhang, XM Lao, K Li, GQ Liao
    PLoS ONE, 2017;12(6):e0179248.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Scaling of titanium implants entrains inflammation-induced osteolysis
    Authors: M Eger, N Sterer, T Liron, D Kohavi, Y Gabet
    Sci Rep, 2017;7(0):39612.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. A novel phthalimide derivative, TC11, has preclinical effects on high-risk myeloma cells and osteoclasts.
    Authors: Matsushita M, Ozaki Y, Hasegawa Y, Terada F, Tabata N, Shiheido H, Yanagawa H, Oikawa T, Matsuo K, Du W, Yamada T, Hozumi M, Ichikawa D, Hattori Y
    PLoS ONE, 2015;10(1):e0116135.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. WNT1-induced Secreted Protein-1 (WISP1), a Novel Regulator of Bone Turnover and Wnt Signaling.
    Authors: Maeda A, Ono M, Holmbeck K, Li L, Kilts T, Kram V, Noonan M, Yoshioka Y, McNerny E, Tantillo M, Kohn D, Lyons K, Robey P, Young M
    J Biol Chem, 2015;290(22):14004-18.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. FTY720 inhibited proinflammatory cytokine release and osteoclastogenesis induced by Aggregatibacter actinomycetemcomitans.
    Authors: Yu H, Herbert B, Valerio M, Yarborough L, Hsu L, Argraves K
    Lipids Health Dis, 2015;14(0):66.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  8. Porphyromonas gingivalis Stimulates Bone Resorption by Enhancing RANKL (Receptor Activator of NF-kappaB Ligand) through Activation of Toll-like Receptor 2 in Osteoblasts.
    Authors: Kassem A, Henning P, Lundberg P, Souza P, Lindholm C, Lerner U
    J Biol Chem, 2015;290(33):20147-58.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. 5-Azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival.
    Authors: Maruyama K, Fukasaka M, Uematsu S, Takeuchi O, Kondo T, Saitoh T, Martino M, Akira S
    J Biol Chem, 2015;290(15):9377-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  10. Anti-osteoclastogenic activity of praeruptorin A via inhibition of p38/Akt-c-Fos-NFATc1 signaling and PLCgamma-independent Ca2+ oscillation.
    Authors: Yeon J, Kim K, Choi S, Moon S, Park Y, Ryu B, Oh J, Kim M, Erkhembaatar M, Son Y, Kim S
    PLoS ONE, 2014;9(2):e88974.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Modulation of osteoclastogenesis with macrophage M1- and M2-inducing stimuli.
    Authors: Jeganathan S, Fiorino C, Naik U, Sun H, Harrison R
    PLoS ONE, 2014;9(8):e104498.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  12. Effects of the peroxisome proliferator-activated receptor (PPAR)-delta agonist GW501516 on bone and muscle in ovariectomized rats.
    Authors: Mosti M, Stunes A, Ericsson M, Pullisaar H, Reseland J, Shabestari M, Eriksen E, Syversen U
    Endocrinology, 2014;155(6):2178-89.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  13. Bisphosphonates inhibit osteosarcoma-mediated osteolysis via attenuation of tumor expression of MCP-1 and RANKL.
    Authors: Ohba T, Cole H, Cates J, Slosky D, Haro H, Ando T, Schwartz H, Schoenecker J
    J Bone Miner Res, 2014;29(6):1431-45.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  14. Reactive oxygen species induce the association of SHP-1 with c-Src and the oxidation of both to enhance osteoclast survival.
    Authors: Ke K, Sul O, Choi E, Safdar A, Kim E, Choi H
    Am J Physiol Endocrinol Metab, 2014;307(1):E61-70.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  15. miR-34a blocks osteoporosis and bone metastasis by inhibiting osteoclastogenesis and Tgif2.
    Authors: Krzeszinski J, Wei W, Huynh H, Jin Z, Wang X, Chang T, Xie X, He L, Mangala L, Lopez-Berestein G, Sood A, Mendell J, Wan Y
    Nature, 2014;512(7515):431-5.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  16. OX40L blockade is therapeutic in arthritis, despite promoting osteoclastogenesis.
    Authors: Gwyer Findlay, Emily, Danks, Lynett, Madden, Jodie, Cavanagh, Mary M, McNamee, Kay, McCann, Fiona, Snelgrove, Robert J, Shaw, Stevan, Feldmann, Marc, Taylor, Peter Ch, Horwood, Nicole J, Hussell, Tracy
    Proc Natl Acad Sci U S A, 2014;111(6):2289-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  17. DLX3 regulates bone mass by targeting genes supporting osteoblast differentiation and mineral homeostasis in vivo.
    Authors: Isaac, J, Erthal, J, Gordon, J, Duverger, O, Sun, H-W, Lichtler, A C, Stein, G S, Lian, J B, Morasso, M I
    Cell Death Differ, 2014;21(9):1365-76.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. (+)-Vitisin A inhibits osteoclast differentiation by preventing TRAF6 ubiquitination and TRAF6-TAK1 formation to suppress NFATc1 activation.
    Authors: Chiou W, Huang Y, Liu Y
    PLoS ONE, 2014;9(2):e89159.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  19. SHIP1 regulates MSC numbers and their osteolineage commitment by limiting induction of the PI3K/Akt/beta-catenin/Id2 axis.
    Authors: Iyer S, Viernes D, Chisholm J, Margulies B, Kerr W
    Stem Cells Dev, 2014;23(19):2336-51.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  20. Comprehensive profiling analysis of actively resorbing osteoclasts identifies critical signaling pathways regulated by bone substrate.
    Authors: Purdue, P Edward, Crotti, Tania N, Shen, Zhenxin, Swantek, Jennifer, Li, Jun, Hill, Jonathan, Hanidu, Adedayo, Dimock, Janice, Nabozny, Gerald, Goldring, Steven R, McHugh, Kevin P
    Sci Rep, 2014;4(0):7595.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  21. Microtubule dynamic instability controls podosome patterning in osteoclasts through EB1, cortactin, and Src.
    Authors: Biosse Duplan, Martin, Zalli, Detina, Stephens, Sebastie, Zenger, Serhan, Neff, Lynn, Oelkers, J Margit, Lai, Frank P, Horne, William, Rottner, Klemens, Baron, Roland
    Mol Cell Biol, 2014;34(1):16-29.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  22. The transcription factor T-box 3 regulates colony-stimulating factor 1-dependent Jun dimerization protein 2 expression and plays an important role in osteoclastogenesis.
    Authors: Yao C, Yao G, Sun B, Zhang C, Tommasini S, Insogna K
    J Biol Chem, 2014;289(10):6775-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  23. Silent information regulator (Sir)T1 inhibits NF-kappaB signaling to maintain normal skeletal remodeling.
    Authors: Edwards J, Perrien D, Fleming N, Nyman J, Ono K, Connelly L, Moore M, Lwin S, Yull F, Mundy G, Elefteriou F
    J Bone Miner Res, 2014;28(4):960-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  24. FoxO proteins restrain osteoclastogenesis and bone resorption by attenuating H2O2 accumulation.
    Authors: Bartell, Shoshana, Kim, Ha-Neui, Ambrogini, Elena, Han, Li, Iyer, Srividhy, Serra Ucer, S, Rabinovitch, Peter, Jilka, Robert L, Weinstein, Robert S, Zhao, Haibo, O'Brien, Charles, Manolagas, Stavros, Almeida, Maria
    Nat Commun, 2014;5(0):3773.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  25. Caspase-2 maintains bone homeostasis by inducing apoptosis of oxidatively-damaged osteoclasts.
    Authors: Sharma, Ramaswam, Callaway, Danielle, Vanegas, Difernan, Bendele, Michelle, Lopez-Cruzan, Marisa, Horn, Diane, Guda, Teja, Fajardo, Roberto, Abboud-Werner, Sherry, Herman, Brian
    PLoS ONE, 2014;9(4):e93696.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  26. Overexpression of developmentally regulated GTP-binding protein-2 increases bone loss.
    Authors: Ke K, Sul O, Kim W, Lee M, Ko M, Suh J, Kim H, Kim S, Park J, Choi H
    Am J Physiol Endocrinol Metab, 2013;304(7):E703-10.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  27. An inducible, ligand-independent receptor activator of NF-kappaB gene to control osteoclast differentiation from monocytic precursors.
    Authors: Rementer C, Wu M, Buranaphatthana W, Yang H, Scatena M, Giachelli C
    PLoS ONE, 2013;8(12):e84465.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  28. RNA interference-mediated silencing of Atp6i prevents both periapical bone erosion and inflammation in the mouse model of endodontic disease.
    Authors: Ma, Junqing, Chen, Wei, Zhang, Lijie, Tucker, Byron, Zhu, Guochun, Sasaki, Hajime, Hao, Liang, Wang, Lin, Ci, Honglian, Jiang, Hongbing, Stashenko, Philip, Li, Yi-Ping
    Infect Immun, 2013;81(4):1021-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  29. Porphyromonas gingivalis infection-associated periodontal bone resorption is dependent on receptor activator of NF-kappaB ligand.
    Authors: Han X, Lin X, Yu X, Lin J, Kawai T, LaRosa K, Taubman M
    Infect Immun, 2013;81(5):1502-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  30. Liver X receptor activation inhibits osteoclastogenesis by suppressing NF-kappaB activity and c-Fos induction and prevents inflammatory bone loss in mice.
    Authors: Kim H, Yoon K, Yoon H, Hong J, Lee M, Lee I, Kim S
    J Leukoc Biol, 2013;94(1):99-107.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  31. Nicotine affects bone resorption and suppresses the expression of cathepsin K, MMP-9 and vacuolar-type H(+)-ATPase d2 and actin organization in osteoclasts.
    Authors: Tanaka H, Tanabe N, Kawato T, Nakai K, Kariya T, Matsumoto S, Zhao N, Motohashi M, Maeno M
    PLoS ONE, 2013;8(3):e59402.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  32. Mitogen-activated protein kinase 2 regulates physiological and pathological bone turnover.
    Authors: Braun T, Lepper J, Ruiz Heiland G, Hofstetter W, Siegrist M, Lezuo P, Gaestel M, Rumpler M, Thaler R, Klaushofer K, Distler J, Schett G, Zwerina J
    J Bone Miner Res, 2013;28(4):936-47.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  33. Inflammatory arthritis increases mouse osteoclast precursors with myeloid suppressor function.
    Authors: Charles, Julia F, Hsu, Lih-Yun, Niemi, Erene C, Weiss, Arthur, Aliprantis, Antonios, Nakamura, Mary C
    J Clin Invest, 2012;122(12):4592-605.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  34. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012;109(25):9971-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  35. Osteoclast inhibitory peptide-1 binding to the Fc gammaRIIB inhibits osteoclast differentiation.
    Authors: Shanmugarajan S, Beeson CC, Reddy SV
    Endocrinology, 2010;151(9):4389-99.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  36. Transient overexpression of sonic hedgehog alters the architecture and mechanical properties of trabecular bone.
    Authors: Kiuru M, Solomon J, Ghali B, van der Meulen M, Crystal RG, Hidaka C
    J. Bone Miner. Res., 2009;24(9):1598-607.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  37. Substance P stimulates bone marrow stromal cell osteogenic activity, osteoclast differentiation, and resorption activity in vitro.
    Authors: Wang L, Zhao R, Shi X, Wei T, Halloran BP, Clark DJ, Jacobs CR, Kingery WS
    Bone, 2009;45(2):309-20.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  38. TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y
    Biochem. Biophys. Res. Commun., 2009;384(3):296-300.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  39. Bidirectional signaling through ephrinA2-EphA2 enhances osteoclastogenesis and suppresses osteoblastogenesis.
    Authors: Irie N, Takada Y, Watanabe Y, Matsuzaki Y, Naruse C, Asano M, Iwakura Y, Suda T, Matsuo K
    J. Biol. Chem., 2009;284(21):14637-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  40. Cardiotrophin-1 is an osteoclast-derived stimulus of bone formation required for normal bone remodeling.
    Authors: Walker EC, McGregor NE, Poulton IJ, Pompolo S, Allan EH, Quinn JM, Gillespie MT, Martin TJ, Sims NA
    J. Bone Miner. Res., 2008;23(12):2025-32.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  41. Osteoprotegerin reduces the serum level of receptor activator of NF-kappaB ligand derived from osteoblasts.
    Authors: Nakamichi Y, Udagawa N, Kobayashi Y, Nakamura M, Yamamoto Y, Yamashita T, Mizoguchi T, Sato M, Mogi M, Penninger JM, Takahashi N
    J. Immunol., 2007;178(1):192-200.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  42. Glucocorticoids act directly on osteoclasts to increase their life span and reduce bone density.
    Authors: Jia D, O'Brien CA, Stewart SA, Manolagas SC, Weinstein RS
    Endocrinology, 2006;147(12):5592-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  43. IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NF-kappa B ligand/osteoprotegerin balance.
    Authors: Lubberts E, van den Bersselaar L, Oppers-Walgreen B, Schwarzenberger P, Coenen-de Roo CJ, Kolls JK, Joosten LA, van den Berg WB
    J. Immunol., 2003;170(5):2655-62.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
Expand to show all 43 Citations
Primary Antibodies
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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ICFlow IC050A  
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WB BAM050 4  
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His Tag Antibody

WB, Flow, AP MAB050R  
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ICFlow IC050G  
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Histidine-tagged Protein Purification Resin

IP999 1
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