Recombinant Mouse TREM-1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1187-TR-025
Product Details
Citations (3)
FAQs
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Recombinant Mouse TREM-1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to block anti-TREM-1-induced TNF-alpha secretion by P388D1 mouse lymphoma cells. The ED50 for this effect is 2-8 ng/mL in the presence of 0.5-2 µg/mL of Goat Anti-Mouse TREM-1 (Catalog # AF1187).
Source
Mouse myeloma cell line, NS0-derived mouse TREM-1 protein
Mouse TREM-1
(Ala21-Ser202)
Accession # Q9JKE2
SIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
47 kDa (monomer)
SDS-PAGE
60-73 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1187-TR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

References
  1. Bouchon, A. (2000) J. Immunol. 164:4991.
  2. Bouchon, A. (2001) Nature 410:1103.
  3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs
54210 (Human); 58217 (Mouse)
Alternate Names
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1

Citations for Recombinant Mouse TREM-1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Enhanced expression of TREM-1 in splenic cDCs in lupus prone mice and it was modulated by miRNA-150
    Authors: Sheng Gao
    Mol. Immunol, 2017;81(0):127-134.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. TREM-1 amplifies corneal inflammation after Pseudomonas aeruginosa infection by modulating Toll-like receptor signaling and Th1/Th2-type immune responses.
    Authors: Wu M, Peng A, Sun M, Deng Q, Hazlett L, Yuan J, Liu X, Gao Q, Feng L, He J, Zhang P, Huang X
    Infect Immun, 2011;79(7):2709-16.
    Species: Mouse
    Sample Types:
    Applications: Bioassay
  3. A role for TREM2 ligands in the phagocytosis of apoptotic neuronal cells by microglia.
    Authors: Hsieh CL, Koike M, Spusta SC, Niemi EC, Yenari M, Nakamura MC, Seaman WE
    J. Neurochem., 2009;109(4):1144-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Primary Antibodies

Mouse TREM-1 Antibody

WB,Flow,AgAct,CyTOF-ready,ICC

Reconstitution Buffers

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