Mouse TREM-1 Antibody
Mouse TREM-1 Antibody Summary
Ala21-Ser202
Accession # Q9JKE2
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Applications
The ED50 for this effect is typically 1‑3 μg/mL.
Mouse TREM-1 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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TREM‑1 in Mouse Splenocytes. TREM-1 was detected in immersion fixed mouse splenocytes treated with LPS (left panel) or untreated (right panel) using Goat Anti-Mouse TREM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1187) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREM-1
TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.
- Bouchon, A. (2000) J. Immunol. 164:4991.
- Bouchon, A. (2001) Nature 410:1103.
- Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Product Datasheets
Citations for Mouse TREM-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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TREM-1 amplifies corneal inflammation after Pseudomonas aeruginosa infection by modulating Toll-like receptor signaling and Th1/Th2-type immune responses.
Authors: Wu M, Peng A, Sun M, Deng Q, Hazlett L, Yuan J, Liu X, Gao Q, Feng L, He J, Zhang P, Huang X
Infect Immun, 2011;79(7):2709-16.
Species: Mouse
Sample Types:
Applications: Bioassay -
TREM-1--expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases.
Authors: Schenk M, Bouchon A, Seibold F, Mueller C
J. Clin. Invest., 2007;117(10):3097-106.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
FAQs
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