Recombinant Mouse USAG1 Protein, CF Summary
Phe24-Ser206, with an N-terminal Met
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in HCl.|
|Reconstitution||Reconstitute at 250 μg/mL in 4 mM HCl.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
USAG1, also known as WISE, Ectodin, or SOSTDC1, is a secreted, monomeric 26-32 kDa glycoprotein of the sclerostin family of BMP antagonists. It functions as a BMP and Wnt antagonist during the development of kidney, tooth, and mammary tissues (1-3). Mature mouse USAG1 contains one cystine knot domain and shares 97% and 99.5% aa identity with human and rat USAG1, respectively (2, 3). USAG1 co-localizes with BMP-7 in the developing and adult kidney, particularly in distal tubule epithelial cells (2, 4). It is also found in ectodermal tissues such as tooth ameloblasts, osteoblasts, cells of the dermal papilla, mammary placode epithelium, and uterine lumenal epithelium (1, 2, 5-7). USAG1 binds BMP-2, -4, -6, and -7, sequestering BMPs and preventing their interaction with BMP receptors (1-4, 8). USAG1 also binds to LRP5 and LRP6 and blocks LRP engagement of Wnt (3, 9). USAG1 coordinates BMP, Wnt, FGF, and Shh signals to regulate apoptosis during tooth and bone development (1, 5, 9, 10). Deletion of USAG1 in mice results in supernumerary teeth, nipples, and whiskers as well as increased bone mineral density (5, 6, 8, 9, 11), while concurrent decreased expression of LRP5 and LRP6 restores normal tooth configurations (9). USAG1-/- mice also show decreased susceptibility to kidney injury which is reversed by neutralizing antibody to BMP-7 (4). In a mouse model of Alport syndrome, deletion of USAG1 ameliorates the loss of renal function (10).
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