Recombinant Human USAG1 Protein, CF

Catalog # Availability Size / Price Qty
5370-SD-050
Product Details
Citations (1)
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Recombinant Human USAG1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

When Recombinant Human (rh) USAG1 is coated at 2 μg/mL (100 μL/well), the concentration of rhLRP-6 Fc Chimera (Catalog # 1505-LR) that produces 50% of the optimal binding response is found to be approximately 0.4‑2 μg/mL.

Source
E. coli-derived human USAG1 protein
Phe24-Ser206, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
20.9 kDa
SDS-PAGE
21 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5370-SD

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USAG1

USAG1 (uterine sensitization‑associated gene 1), also called WISE (Wnt modulator in surface ectoderm) or Ectodin, gene name SOSTDC1, is a secreted, monomeric 26 ‑ 32 kDa glycoprotein of the sclerostin family of BMP antagonists (1 ‑ 3). Human USAG1 cDNA encodes 206 amino acids (aa), including a 23 aa signal sequence and a 183 aa mature protein with a cystine knot domain (2, 3). Mature human USAG1 shares 96 ‑ 98% aa identity with mouse, rat, canine, equine, porcine and bovine USAG1. USAG1 co‑localizes with BMP7 in the developing and adult kidney, especially in renal distal tubule cells postnatally (2, 4). It is also found in ectodermal tissues such as ameloblasts of teeth (for enamel formation), and cells of the dermal papilla (1, 2). USAG1 binds BMP‑2, ‑4, ‑6, and ‑7, sequestering BMPs and making them unavailable for BMP receptor binding (1 ‑ 5). USAG1 also binds LRPs such as LRP6, blocking LRP6 engagement of Wnt (3, 6). In dental mesenchymal cells, USAG1 is proposed to coordinate BMP, Wnt, FGF and SHH signals that regulate apoptosis during tooth development (1, 6, 7). Deletion of USAG1 results in mice with supernumerary teeth, while concurrent decreased expression of LRP5 and LRP6 restores normal tooth configurations (4 ‑ 6, 8).  USAG1-/- mice show decreased susceptibility to kidney injury which is reversed by administration of a neutralizing antibody to BMP7 (4). In a mouse model of Alport syndrome, deletion of USAG1 alternates disease progression (7). These results support a proposed USAG1/WISE antagonism of BMP and Wnt signaling pathways in kidney and tooth development. USAG1 expression by uterine luminal epithelium is also associated with the timing and positioning of blastocyst implantation (9).

References
  1. Laurikkala, J. et al. (2003) Dev. Biol. 264:91.
  2. Yanagita, M. et al. (2004) Biochem. Biophys. Res. Commun. 316:490.
  3. Lintern, K.B. et al. (2009) J. Biol. Chem. 284:23159.
  4. Yanagita, M. et al. (2006) J. Clin.  Invest. 116:70.
  5. Murashima-Suginami, A. et al. (2008) Biochem. Biophys. Res. Commun. 369:1012.
  6. Ahn, Y. et al. (2010) Development 137:3221.
  7. Tanaka, M. et al. (2010) J. Clin. Invest. 120:768.
  8. Kassai, Y. et al. (2005) Science 309:2067.
  9. Maeda, K. et al. (2007) J. Reprod. Dev. 53:931.
Long Name
Sclerostin Domain Containing Protein 1/Uterine Sensitization-associated Gene 1 Protein
Entrez Gene IDs
25928 (Human); 66042 (Mouse); 266803 (Rat)
Alternate Names
CDA019; cystine-knot containing secreted protein; DKFZp564D206; Ectodermal BMP inhibitor; Ectodin; sclerostin domain containing 1; sclerostin domain-containing protein 1; SOSTDC1; USAG1; USAG-1; USAG1uterine sensitization-associated protein-1; Uterine sensitization-associated gene 1 protein; WISE

Citation for Recombinant Human USAG1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Hepcidin regulation in prostate and its disruption in prostate cancer.
    Authors: Tesfay L, Clausen K, Kim J, Hegde P, Wang X, Miller L, Deng Z, Blanchette N, Arvedson T, Miranti C, Babitt J, Lin H, Peehl D, Torti F, Torti S
    Cancer Res, 2015;75(11):2254-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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