Recombinant Human USAG1 Protein, CF Summary
When Recombinant Human (rh) USAG1 is coated at 2 μg/mL (100 μL/well), the concentration of rhLRP-6 Fc Chimera (Catalog # 1505-LR) that produces 50% of the optimal binding response is found to be approximately 0.4‑2 μg/mL.
Phe24-Ser206, with an N-terminal Met
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in HCl.|
|Reconstitution||Reconstitute at 250 μg/mL in 4 mM HCl.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
USAG1 (uterine sensitization‑associated gene 1), also called WISE (Wnt modulator in surface ectoderm) or Ectodin, gene name SOSTDC1, is a secreted, monomeric 26 ‑ 32 kDa glycoprotein of the sclerostin family of BMP antagonists (1 ‑ 3). Human USAG1 cDNA encodes 206 amino acids (aa), including a 23 aa signal sequence and a 183 aa mature protein with a cystine knot domain (2, 3). Mature human USAG1 shares 96 ‑ 98% aa identity with mouse, rat, canine, equine, porcine and bovine USAG1. USAG1 co‑localizes with BMP7 in the developing and adult kidney, especially in renal distal tubule cells postnatally (2, 4). It is also found in ectodermal tissues such as ameloblasts of teeth (for enamel formation), and cells of the dermal papilla (1, 2). USAG1 binds BMP‑2, ‑4, ‑6, and ‑7, sequestering BMPs and making them unavailable for BMP receptor binding (1 ‑ 5). USAG1 also binds LRPs such as LRP6, blocking LRP6 engagement of Wnt (3, 6). In dental mesenchymal cells, USAG1 is proposed to coordinate BMP, Wnt, FGF and SHH signals that regulate apoptosis during tooth development (1, 6, 7). Deletion of USAG1 results in mice with supernumerary teeth, while concurrent decreased expression of LRP5 and LRP6 restores normal tooth configurations (4 ‑ 6, 8). USAG1-/- mice show decreased susceptibility to kidney injury which is reversed by administration of a neutralizing antibody to BMP7 (4). In a mouse model of Alport syndrome, deletion of USAG1 alternates disease progression (7). These results support a proposed USAG1/WISE antagonism of BMP and Wnt signaling pathways in kidney and tooth development. USAG1 expression by uterine luminal epithelium is also associated with the timing and positioning of blastocyst implantation (9).
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Citation for Recombinant Human USAG1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Hepcidin regulation in prostate and its disruption in prostate cancer.
Authors: Tesfay L, Clausen K, Kim J, Hegde P, Wang X, Miller L, Deng Z, Blanchette N, Arvedson T, Miranti C, Babitt J, Lin H, Peehl D, Torti F, Torti S
Cancer Res, 2015;75(11):2254-63.
Sample Types: Whole Cells
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