Recombinant Mouse VEGF 188 Protein

Formulations:
Catalog # Availability Size / Price Qty
7916-MV-010
Product Details
Citations (1)
FAQs
Reviews

Recombinant Mouse VEGF 188 Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 7-35 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived mouse VEGF protein
Ala27-Arg214
Accession #
N-terminal Sequence
Analysis
Ala27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
22.1 kDa (monomer)
SDS-PAGE
28-30 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7916-MV

Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7916-MV/CF

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF

Vascular endothelial growth factor (VEGF or VEGF‑A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1‑3). It is a member of the PDGF family that is characterized by a cystine knot structure formed by eight conserved cysteine residues (4). Alternate splicing produces isoforms including 121, 145, 165, 183, 189, and 206 amino acid (aa) forms in humans, with 120, 164 and 188 aa isoforms found in mouse (1‑4). Mouse VEGF188 shares 98% aa sequence identity with the appropriate isoform in rat, and 89% with human, bovine and canine VEGF. While isoforms VEGF120 and VEGF121 are freely diffusible, VEGF188, VEGF189 and VEGF206 contain the highest number of basic aa, which bind heparin and tether these isoforms to the cell surface and extracellular matrix (3‑5). Expression of VEGF188/189 is particularly high in the embryonic lung, where it is produced by type II alveolar epithelia (6). It is thought to be involved in lung, heart and liver vasculogenesis (5, 6). It is not sufficient for vasculogenesis during bone development, but may play a role in bone repair (5, 7). Tumor cell production of VEGF188/189 correlates with poor prognosis (5). VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt‑1) and VEGF R2 (Flk‑1/KDR) on endothelial cells (4). Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF188 binds VEGF R2 best when it is cleaved by uPA or plasmin into a 110‑111 aa form (4, 5). Human VEGF165 and VEGF189 also bind the semaphorin receptor Neuropilin‑1 (8, 9).

References
  1. Breier, G. et al. (1992) Development 114:521.
  2. Shima, D.T. et al. (1996) J. Biol. Chem. 271:3877.
  3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.
  4. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.
  5. Woolard, J. et al. (2009) Microcirculation 16:572.
  6. Ng, Y.S. et al. (2001) Dev. Dyn. 220:112.
  7. Maes, C. et al. (2004) J. Clin. Endocrinol. 113:188.
  8. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049.
  9. Vintonenko, N. et al. (2011) Cell Adh. Migr. 5:332.
Long Name
Vascular Endothelial Growth Factor
Entrez Gene IDs
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

Citation for Recombinant Mouse VEGF 188 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Alkaloid extract of Corydalis yanhusuo inhibits angiogenesis via targeting vascular endothelial growth factor receptor signaling
    Authors: L Wan, Y Zhao, Q Zhang, G Gao, S Zhang, Y Gao, X Chen, X Qian
    BMC Complement Altern Med, 2019;19(1):359.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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