Recombinant Rat beta-NGF Protein, CF

A New and Improved rrb-NGF is Now Available! It is CHO expressed and has a lower endotoxin spec!
New Product 7815-NG/CF.
(11 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.1-0.6 ng/mL.
  • Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived Ser122-Gly241
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser122
  • Predicted Molecular Mass
    13.2 kDa (monomer)
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
556-NG/CF
 
556-NG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Rat beta -NGF (Catalog # 556‑NG/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.1-0.6 ng/mL.
1 µg/lane of Recombinant Rat beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 12 kDa.
Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland over three decades ago as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta  subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant rat beta -NGF is a homodimer of two 120-amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotrophic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

  • Long Name:
    beta Nerve Growth Factor
  • Entrez Gene IDs:
    4803 (Human); 18049 (Mouse); 310738 (Rat)
  • Alternate Names:
    Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Requirement of cAMP signaling for Schwann cell differentiation restricts the onset of myelination.
    Authors: Bacallao, Ketty, Monje, Paula V
    PLoS ONE, 2015;10(2):e0116948.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  2. Intermittent hypoxia-induced protein phosphatase 2A activation reduces PC12 cell proliferation and differentiation.
    Authors: Chen T, Chiu H, Pan Y, Hsu S, Lin J, Yang K
    J Biomed Sci, 2014;21(0):46.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  3. Incorporation of chitosan microspheres into collagen-chitosan scaffolds for the controlled release of nerve growth factor.
    Authors: Zeng, Wen, Rong, Mengyao, Hu, Xueyu, Xiao, Wei, Qi, Fengyu, Huang, Jinghui, Luo, Zhuojing
    PLoS ONE, 2014;9(7):e101300.
    Species: N/A
    Sample Type: Beads
  4. Opposing roles of PKA and EPAC in the cAMP-dependent regulation of schwann cell proliferation and differentiation [corrected].
    Authors: Bacallao, Ketty, Monje, Paula V
    PLoS ONE, 2013;8(12):e82354.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  5. LDL receptor-related protein-1 is a sialic-acid-independent receptor for myelin-associated glycoprotein that functions in neurite outgrowth inhibition by MAG and CNS myelin.
    Authors: Stiles, Travis L, Dickendesher, Travis L, Gaultier, Alban, Fernandez-Castaneda, Anthony, Mantuano, Elisabet, Giger, Roman J, Gonias, Steven L
    J Cell Sci, 2013;126(0):209-20.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  6. Rab7 silencing prevents mu-opioid receptor lysosomal targeting and rescues opioid responsiveness to strengthen diabetic neuropathic pain therapy.
    Authors: Mousa S, Shaqura M, Khalefa B, Zollner C, Schaad L, Schneider J, Shippenberg T, Richter J, Hellweg R, Shakibaei M, Schafer M
    Diabetes, 2013;62(4):1308-19.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  7. Contribution of nerve growth factor to augmented TRPV1 responses of muscle sensory neurons by femoral artery occlusion.
    Authors: Xing J, Lu J, Li J
    Am. J. Physiol. Heart Circ. Physiol., 2009;296(5):H1380-7.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  8. Signal transduction mechanism involved in Clostridium perfringens alpha-toxin-induced superoxide anion generation in rabbit neutrophils.
    Authors: Oda M, Ikari S, Matsuno T, Morimune Y, Nagahama M, Sakurai J
    Infect. Immun., 2006;74(5):2876-86.
    Species: Rabbit
    Sample Type: Whole Cells
    Application: Bioassay
  9. TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury.
    Authors: Obata K, Katsura H, Mizushima T, Yamanaka H, Kobayashi K, Dai Y, Fukuoka T, Tokunaga A, Tominaga M, Noguchi K
    J. Clin. Invest., 2005;115(9):2393-401.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  10. Differential activation of MAPK in injured and uninjured DRG neurons following chronic constriction injury of the sciatic nerve in rats.
    Authors: Obata K, Yamanaka H, Dai Y, Mizushima T, Fukuoka T, Tokunaga A, Noguchi K
    Eur. J. Neurosci., 2004;20(11):2881-95.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  11. Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes.
    Authors: Nevin Z, Factor D, Karl R, Douvaras P, Laukka J, Windrem M, Goldman S, Fossati V, Hobson G, Tesar P
    Am J Hum Genet, 0;100(4):617-634.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 11 Citations

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