Recombinant Rat beta-NGF Protein

Newer Version Available: 7815-NG
NEW
A New and Improved rr b-NGF is Now Available! It is CHO expressed and has a lower endotoxin specification!

Carrier Free

Catalog # Availability Size / Price Qty
556-NG-100/CF

With Carrier

Catalog # Availability Size / Price Qty
556-NG-100
Recombinant Rat beta-NGF Protein Bioactivity
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Product Details
Citations (23)
FAQs
Reviews (3)

Recombinant Rat beta-NGF Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.1-0.6 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat beta-NGF protein
Ser122-Gly241
Accession #
N-terminal Sequence
Analysis
Ser122
Predicted Molecular Mass
13.2 kDa (monomer)

Product Datasheets

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556-NG (with carrier)

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556-NG/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

556-NG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

556-NG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Rat beta-NGF Protein Bioactivity View Larger

Recombinant Rat beta -NGF (Catalog # 556‑NG) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.1-0.6 ng/mL.

SDS-PAGE Recombinant Rat beta-NGF Protein SDS-PAGE View Larger

1 µg/lane of Recombinant Rat beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 12 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland over three decades ago as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta  subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant rat beta -NGF is a homodimer of two 120-amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotrophic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Long Name
beta Nerve Growth Factor
Entrez Gene IDs
4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)
Alternate Names
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428

Citations for Recombinant Rat beta-NGF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Neurite Outgrowth-Promoting Compounds from Cockscomb Hydrolysate
    Authors: T Koga, A Tai
    Nutrients, 2022;14(7):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. 3D micro-organisation printing of mammalian cells to generate biological tissues
    Authors: GDM Jeffries, S Xu, T Lobovkina, V Kirejev, F Tusseau, C Gyllensten, AK Singh, P Karila, L Moll, O Orwar
    Sci Rep, 2020;10(1):19529.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Anti-IL-20 antibody improved motor function and reduced glial scar formation after traumatic spinal cord injury in rats
    Authors: JS Lee, YH Hsu, YS Chiu, IM Jou, MS Chang
    J Neuroinflammation, 2020;17(1):156.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Ameliorative effects of aqueous extract of Forsythiae suspensa fruits on oxaliplatin-induced neurotoxicity in vitro and in vivo
    Authors: JM Yi, S Shin, NS Kim, OS Bang
    BMC Complement Altern Med, 2019;19(1):339.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. Preparation and evaluation of microfluidic magnetic alginate microparticles for magnetically templated hydrogels
    Authors: I Singh, CS Lacko, Z Zhao, CE Schmidt, C Rinaldi
    J Colloid Interface Sci, 2019;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Neuroprotective Effects of an Aqueous Extract of Forsythia viridissima and Its Major Constituents on Oxaliplatin-Induced Peripheral Neuropathy
    Authors: JM Yi, S Shin, NS Kim, OS Bang
    Molecules, 2019;24(6):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Weak power frequency magnetic fields induce microtubule cytoskeleton reorganization depending on the epidermal growth factor receptor and the calcium related signaling.
    Authors: Xia Wu, Juan Du, Weitao Song, Meiping Cao, Shude Chen, Ruohong Xia
    PLoS ONE, 2018;0(0):1932-6203.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Teriflunomide promotes oligodendroglial differentiation and myelination
    Authors: P Göttle, A Manousi, D Kremer, L Reiche, HP Hartung, P Küry
    J Neuroinflammation, 2018;15(1):76.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Differential contributions of peripheral and central mechanisms to pain in a rodent model of osteoarthritis
    Authors: AR Haywood, GJ Hathway, V Chapman
    Sci Rep, 2018;8(1):7122.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  10. Pterostilbene inhibits reactive oxygen species production and apoptosis in primary spinal cord neurons by activating autophagy via the mechanistic target of rapamycin signaling pathway
    Authors: JL He, XH Dong, ZH Li, XY Wang, ZA Fu, N Shen
    Mol Med Rep, 2018;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes
    Authors: ZS Nevin, DC Factor, RT Karl, P Douvaras, J Laukka, MS Windrem, SA Goldman, V Fossati, GM Hobson, PJ Tesar
    Am. J. Hum. Genet., 2017;100(4):617-634.
    Applications: Bioassay
  12. The TrkA receptor mediates experimental thermal hyperalgesia produced by nerve growth factor: modulation by the p75 neurotrophin receptor
    Authors: Gary Strichartz
    Neuroscience, 2016;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  13. Requirement of cAMP signaling for Schwann cell differentiation restricts the onset of myelination.
    Authors: Bacallao, Ketty, Monje, Paula V
    PLoS ONE, 2015;10(2):e0116948.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Incorporation of chitosan microspheres into collagen-chitosan scaffolds for the controlled release of nerve growth factor.
    Authors: Zeng, Wen, Rong, Mengyao, Hu, Xueyu, Xiao, Wei, Qi, Fengyu, Huang, Jinghui, Luo, Zhuojing
    PLoS ONE, 2014;9(7):e101300.
    Species: N/A
    Sample Types: Beads
  15. Intermittent hypoxia-induced protein phosphatase 2A activation reduces PC12 cell proliferation and differentiation.
    Authors: Chen T, Chiu H, Pan Y, Hsu S, Lin J, Yang K
    J Biomed Sci, 2014;21(0):46.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  16. LDL receptor-related protein-1 is a sialic-acid-independent receptor for myelin-associated glycoprotein that functions in neurite outgrowth inhibition by MAG and CNS myelin.
    Authors: Stiles, Travis L, Dickendesher, Travis L, Gaultier, Alban, Fernandez-Castaneda, Anthony, Mantuano, Elisabet, Giger, Roman J, Gonias, Steven L
    J Cell Sci, 2013;126(0):209-20.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Opposing roles of PKA and EPAC in the cAMP-dependent regulation of schwann cell proliferation and differentiation [corrected].
    Authors: Bacallao, Ketty, Monje, Paula V
    PLoS ONE, 2013;8(12):e82354.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Rab7 silencing prevents mu-opioid receptor lysosomal targeting and rescues opioid responsiveness to strengthen diabetic neuropathic pain therapy.
    Authors: Mousa S, Shaqura M, Khalefa B, Zollner C, Schaad L, Schneider J, Shippenberg T, Richter J, Hellweg R, Shakibaei M, Schafer M
    Diabetes, 2013;62(4):1308-19.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  19. Contribution of nerve growth factor to augmented TRPV1 responses of muscle sensory neurons by femoral artery occlusion.
    Authors: Xing J, Lu J, Li J
    Am. J. Physiol. Heart Circ. Physiol., 2009;296(5):H1380-7.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  20. Signal transduction mechanism involved in Clostridium perfringens alpha-toxin-induced superoxide anion generation in rabbit neutrophils.
    Authors: Oda M, Ikari S, Matsuno T, Morimune Y, Nagahama M, Sakurai J
    Infect. Immun., 2006;74(5):2876-86.
    Species: Rabbit
    Sample Types: Whole Cells
    Applications: Bioassay
  21. TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury.
    Authors: Obata K, Katsura H, Mizushima T, Yamanaka H, Kobayashi K, Dai Y, Fukuoka T, Tokunaga A, Tominaga M, Noguchi K
    J. Clin. Invest., 2005;115(9):2393-401.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  22. Differential activation of MAPK in injured and uninjured DRG neurons following chronic constriction injury of the sciatic nerve in rats.
    Authors: Obata K, Yamanaka H, Dai Y, Mizushima T, Fukuoka T, Tokunaga A, Noguchi K
    Eur. J. Neurosci., 2004;20(11):2881-95.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  23. Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes.
    Authors: Nevin Z, Factor D, Karl R, Douvaras P, Laukka J, Windrem M, Goldman S, Fossati V, Hobson G, Tesar P
    Am J Hum Genet, 0;100(4):617-634.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Rat beta-NGF Protein

Average Rating: 4.7 (Based on 3 Reviews)

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Recombinant Rat beta-NGF Protein
By Anonymous on 03/13/2020
Application: Tested with ELISA before using in a rat model.

Recombinant Rat beta-NGF Protein
By Anonymous on 01/08/2019
Application: MassSpec characterization.

Recombinant Rat beta-NGF Protein
By Brad Morrison on 01/15/2016
Application: Differentiation assay
Reason for Rating: Differentiated PC12 cells with 100ng/mL of NGF for 7 days. Cells look neuronal.