Recombinant Rat CCL19/MIP-3 beta Protein

Carrier Free

Catalog # Availability Size / Price Qty
7800-M3-025/CF

With Carrier

Catalog # Availability Size / Price Qty
7800-M3-025
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Rat CCL19/MIP-3 beta Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR7. The ED50 for this effect is 2-10 ng/mL.
Source
E. coli-derived rat CCL19/MIP-3 beta protein
Gly26-Ser108
Accession #
N-terminal Sequence
Analysis
Starts at Gly26
Structure / Form
Monomer
Predicted Molecular Mass
9.1 kDa
SDS-PAGE
10 kDa, reducing conditions

Product Datasheets

7800-M3 (with carrier)

7800-M3/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7800-M3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7800-M3/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL19/MIP-3 beta

CCL19 (also known as Mip-3 beta, ELC, CK beta -11, Scya19 and Exodus-3) is a 9 kDa secreted member of the CC family of chemotactic cytokines (1-4). Cells known to express CCL19 are limited in number, and include activated monocytes (5), stromal cells in nodal T zones (6), CD8+ dendritic cells (DC) (6), vascular endothelial cells (7), visceral smooth muscle and mast cells (8), and thymic stromal cells (9).  Rat CCL19 is synthesized as a precursor that is 1108 amino acids (aa) in length. The precursor contains a 25 aa signal sequence plus an 83 aa mature region (aa 26-108) (10). The signature CC chemokine motif occurs at Cys33Cys34 and there are no potential N-linked glycosylation sites. It is unclear if rat CCL19 forms homodimers. Mature rat CCL19 shares 72% and 89% aa sequence identity with human and mouse CCL19, respectively (3, 4, 10).

CCL19 is known to bind to CCR7 (3, 4), CCLR2/CRAM (L-CCR in rodent) (11, 12, 13) and CCX-CKR (14), with the last two receptors representing scavenger, or chemokine-sink receptors. Cells expressing the signaling receptor (CCR7) are varied in type, and include CD56+ NK cells (15), naïve CD4+ T and activated B cells (4), mature bone marrow-derived dendritic and Langerhans cells (16), Collagen I+III+Fibronectin+ fibrocytes (17) and CD4+ Tregs (9). Upon CCR7 engagement, CCL19 has a number of documented effects. In the case of the DC, it reportedly induces DC cytoplasmic extension, increases endocytic activity, protects DC from apoptosis, increases the speed of DC migration, and promotes its secretion of cytokines (18). Notably, while CCL19 is a potent chemoattractant, this activity seems to be dependent upon concomitant EP2 and EP4 receptor activation, coupled to an increase in the presence of NO (18). CCL19 is perhaps best known as a secondary lymphoid organ homing molecule for naïve lymphocytes. Here, a CCL21 gradient is believed to first draw naïve CD4+ T cells into tissue lymphatic channels. At this point, CCL19 becomes predominate, amplifying chemoattraction and inducing an up‑regulation of EDG1, a receptor for sphingosine‑1 phosphate/S1P. Upon entry into the lymph node, the naïve CD4+ T cells encounters APCs/DCs which may, or may not, be presenting compatible antigen. If so, an immunological synapse is generated and the activated T cell remains in the node. If not, the continuous CCL19:CCR7 interaction results in an internalization of CCR7, with a resultant loss of chemoattractive activity. The activity of up‑regulated EDG1 now predominates, and naïve T cells migrate out of the node, and into the blood in response to a constitutive gradient of S1P. The exact source of S1P is unclear, but may represent a natural difference between plasma and tissue levels (19, 20).

References
  1. Sharma, M. (2010) Crit. Rev. Biotechnol. 30:1.
  2. Blanchet, X. et al. (2012) Front. Immunol.3:175.
  3. Yoshida, R. et al. (1997) J. Biol. Chem.272:13803.
  4. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
  5. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
  6. Luther, S.A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12694.
  7. Alt, C. et al. (2002) Eur. J. Immunol. 32:2133.
  8. Kaur, D. et al. (2006) Am. J. Respir. Crit. Care Med. 174:1179.
  9. Takamura, K. et al. (2007) J. Immunol. 179:5897.
  10. GenBank Accession # NP_001102131.
  11. Leick, M. et al. (2009) Immunology 129:536.
  12. Yoshimura, T. & J.J. Oppenheim (2011) Exp. Cell Res. 317:674.
  13. Shimida, T. et al. (1998) FEBS Lett. 425:490.
  14. Comerford, I. et al. (2006) Eur. J. Immunol. 36:1904
  15. Maghazachi, A.A. (2010) Curr. Top. Microbiol. Immunol. 341:37.
  16. Yanagihara, S. et al. (1998) J. Immunol. 161:3096.
  17. Abe, R. et al. (2001) J. Immunol. 166:7556.
  18. Sanchez-Sanchez, N. et al. (2006) J. Immunol. 176:5153.
  19. Shannon, L.A. et al. (2012) J. Biol. Chem. 287:11656.
  20. Schwab, S.R. & J.G. Cyster (2007) Nat. Immunol. 8:1295.
Entrez Gene IDs
6363 (Human); 24047 (Mouse); 362506 (Rat)
Alternate Names
beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19

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