Recombinant Rat CD40 Ligand/TNFSF5 (HA-tag) Protein

Name: Recombinant Rat CD40 Ligand/TNFSF5 (HA-tag) Protein
Brand: R&D Systems
SKU: 8414-CL
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # 8414-CL

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems CHO-derived Recombinant Rat CD40 Ligand/TNFSF5 (HA-tag) Protein (8414-CL)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q9Z2V2

Applications

Bioactivity

View all CD40 Ligand/TNFSF5 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived rat CD40 Ligand/TNFSF5 protein

HA (YPYDVPDYA)	GCN4-IZ	GGGSGGGSGGGS	Rat CD40 Ligand (Met112-Leu260 Accession # Q9Z2V2
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr

Predicted Molecular Mass

22 kDa

SDS-PAGE

22-30 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using mouse splenic B cells in the presence of IL-4. Banchereau, J. et al. (1991) Science 251:70.
The ED₅₀ for this effect is 0.15-0.9 ng/mL in the presence of 0.1 μg/mL of the cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060).

Scientific Data Images for Recombinant Rat CD40 Ligand/TNFSF5 (HA-tag) Protein

Recombinant Rat CD40 Ligand/TNFSF5 Protein SEC-MALS.

Recombinant Rat CD40 Ligand/TNFSF5 (Catalog # 8414-CL) has a molecular weight (MW) of 67.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Recombinant Rat CD40 Ligand/TNFSF5 (HA-tag) Protein Bioactivity

Recombinant Rat CD40 Ligand (Catalog# 8414-CL) induces cell proliferation by mouse splenic B cells in the presence of IL-4. The ED₅₀ for this effect is 0.15-0.9 ng/mL in the presence of 0.1 µg/mL of the cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060).

Recombinant Rat CD40 Ligand/TNFSF5 (HA-tag) Protein SDS-PAGE

1 μg/lane of Recombinant Rat CD40 Ligand/TNFSF5 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing R bands at 26, 28 kDa and NR bands at 26, 28 kDa.

Formulation, Preparation, and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8414-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in steril PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Carrier Free: 8414-CL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in sterile PBS and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD40 Ligand/TNFSF5

CD40 Ligand, also known as TNFSF5, CD154, TRAP, or gp39, is a 33-39 kDa type II transmembrane glycoprotein member of the TNF superfamily (1, 2). Mature rat CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 217 aa extracellular region. The extracellular domain of rat CD40 Ligand shares 75% and 93% amino acid (aa) sequence identity with the human and mouse proteins, respectively. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial cells, and epithelial cells (3). The 18 kDa soluble form (aa 112-260) arises from proteolytic processing and retains the ability to bind and activate CD40 (4, 5). Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes (2). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (6, 7). CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS (8, 9). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (10, 11).