Recombinant Rat CX3CL1/Fractalkine (Chemokine Domain)

Carrier Free

Catalog # Availability Size / Price Qty
537-FT-025/CF

With Carrier

Catalog # Availability Size / Price Qty
537-FT-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (5)
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Reviews (1)

Recombinant Rat CX3CL1/Fractalkine (Chemokine Domain) Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CX3CR1. The ED50 for this effect is 3-18 ng/mL.
Source
E. coli-derived rat CX3CL1/Fractalkine protein
Gln25-Gly100
Accession #
N-terminal Sequence
Analysis
Gln25
Predicted Molecular Mass
8.8 kDa

Product Datasheets

537-FT (with carrier)

537-FT/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

537-FT

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

537-FT/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CX3CL1/Fractalkine

CX3CL1, also named neurotactin, is a member of the delta chemokine subfamily that contains a novel C-X3-C motif. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Rat CX3CL1 cDNA encodes a 393 amino acid (aa) residue precursor protein with two alternative (21 aa or 24 aa residue) putative signal peptides, a 76 aa residue globular chemokine domain, a 238 aa residue stalk region rich in Gly, Pro, Ser and Thr and containing degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 36 aa residue cytoplasmic domain. The extracellular domain of CX3CL1 can potentially be released as a soluble protein by proteolysis at the conserved dibasic motif proximal to the transmembrane region. With the exception of the stalk region, rat CX3CL1 shares a high degree of amino sequence homology (83% sequence identity) with human and mouse CX3CL1. CX3CL1 is expressed in various tissues including heart, brain, lung, kidney, skeletal muscle, and testis. In rat brain, CX3CL1 expression was found to be localized principally to neurons. The expression of CX3CL1 was also reported to be up‑regulated on activated endothelial cells. Membrane‑bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T‑lymphocytes but not monocytes. CX3CR1, previously named V28 or chemokine beta receptor‑like 1, has been found to be a specific receptor for CX3CL1. In addition, US28, a 7TM receptor encoded by human cytomegalovirus that binds multiple CC chemokines, has also been shown to bind fractalkine with high-affinity.

References
  1. Kledal, T.N. et al. (1998) FEBS Lett. 441:209.
  2. Combadiere, C. et al. (1998) J. Biol. Chem. 273:23799.
  3. Harrison, J.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10896.
  4. Rossi, D.L. et al. (1998) Genomics 47:163.
Entrez Gene IDs
6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names
ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Citations for Recombinant Rat CX3CL1/Fractalkine (Chemokine Domain)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathy
    Authors: M Jiang, H Xie, C Zhang, T Wang, H Tian, L Lu, JY Xu, GT Xu, L Liu, J Zhang
    Journal of Cellular and Molecular Medicine, 2022;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: Bioassay
  2. Selective activation of microglia facilitates synaptic strength.
    Authors: Clark A, Gruber-Schoffnegger D, Drdla-Schutting R, Gerhold K, Malcangio M, Sandkuhler J
    J Neurosci, 2015;35(11):4552-70.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Cytokine expression profiling of the myocardium reveals a role for CX3CL1 (fractalkine) in heart failure.
    Authors: Husberg C, Nygard S, Finsen AV, Damas JK, Frigessi A, Oie E, Waehre A, Gullestad L, Aukrust P, Yndestad A, Christensen G
    J. Mol. Cell. Cardiol., 2008;45(2):261-9.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. The chemokine CX3CL1/fractalkine interferes with the antinociceptive effect induced by opioid agonists in the periaqueductal grey of rats.
    Authors: Chen X, Geller EB, Rogers TJ, Adler MW
    Brain Res., 2007;1153(0):52-7.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  5. Role of the CX3CR1/p38 MAPK pathway in spinal microglia for the development of neuropathic pain following nerve injury-induced cleavage of fractalkine.
    Authors: Zhuang ZY, Kawasaki Y, Tan PH, Wen YR, Huang J, Ji RR
    Brain Behav. Immun., 2006;21(5):642-51.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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Reviews for Recombinant Rat CX3CL1/Fractalkine (Chemokine Domain)

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Recombinant Rat CX3CL1/Fractalkine (Chemokine Domain)
By Anonymous on 10/17/2016
Application: Immunoassay Standard