Recombinant Rat Fc gamma RIIA/CD32a Protein, CF

Catalog # Availability Size / Price Qty
9696-CD-100
Recombinant Rat Fc gamma RIIA/CD32a Protein Binding Activity
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Rat Fc gamma RIIA/CD32a Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat Fc gamma  RIIA/CD32a is immobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 1.25-6.25 μg/mL
Source
Human embryonic kidney cell, HEK293-derived rat Fc gamma RIIA/CD32a protein
Asp37-His220, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asp37
Predicted Molecular Mass
22 kDa
SDS-PAGE
29-44 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9696-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Rat Fc gamma RIIA/CD32a Protein Binding Activity View Larger

When Recombinant Rat Fc gamma RIIA/CD32a (Catalog # 9696-CD) is immobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated Rat IgG with an ED50 of 1.25-6.25 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Fc gamma RIIA/CD32a

Receptors for the Fc region of IgG (Fc gamma  Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, cytokine release, and B cell proliferation. There are three classes of Fc gamma  Rs, based on close relationships in their extracellular domains: Fc gamma  RI/CD64, Fc gamma  RII/CD32, and Fc gamma  RIII/CD16. Each group may be encoded by multiple genes and exists in different isoforms depending on species and cell type (1-3). In humans, there are three Fc gamma  RII genes that encode multiple transcripts that differ in their cytoplasmic tails. Only one murine Fc gamma  RII gene has been identified which has two transcripts that are homologues of the human Fc gamma  RIIB (4). Fc gamma  RIIA is not expressed in mice. Approximately 40 kDa, Fc gamma  RIIA is a type 1 transmembrane glycoprotein that is expressed on different cell types, including, neutrophils, monocytes, dendritic cells, and is the only Fc gamma  R expressed on human platelets (5). The mature protein consists of an extracellular domain (ECD), a transmembrane segment, and a cytoplasmic domain. Within the ECD, rat Fc gamma RIIA shares 52% amino acid sequence identity with human Fc gamma RIIA. The Fc gamma  RI/CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the Fc gamma RII/CD32 and Fc gamma  RIII/CD16 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 6). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 7). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (7).

References
  1. Van de Winkel, J. and P. Capel (1993) Immunol. Today. 14:215.
  2. Raghavan, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Ravetch, J.V. and J.P. Kinet (1991) Annu. Rev. Immunol. 9:457.
  5. Arman, M. and K. Krauel (2015) J. Thromb. Haemost. 13:893.
  6. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  7. Ravetch, J. and L. Lanier (2000) Science 290:84.
Long Name
Fc gamma Receptor II A
Entrez Gene IDs
2212 (Human); 14130 (Mouse); 116591 (Rat); 102143568 (Cynomolgus Monkey)
Alternate Names
CD32 antigen; CD32a; CD32MGC23887; CDw32fc-gamma-RIIa; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32); Fc gamma RIIA; FCG2; Fc-gamma RII-a; Fc-gamma-RIIa; FcGR; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; IGFR2MGC30032; IgG Fc receptor II-a; Immunoglobulin G Fc receptor II; low affinity immunoglobulin gamma Fc region receptor II-a

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Rat Fc gamma RIIA/CD32a Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Rat Fc gamma RIIA/CD32a Protein, CF and earn rewards!

Have you used Recombinant Rat Fc gamma RIIA/CD32a Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review