Recombinant Rat Fc gamma RIIA/CD32a Protein, CF
Recombinant Rat Fc gamma RIIA/CD32a Protein, CF Summary
Asp37-His220, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Rat Fc gamma RIIA/CD32a (Catalog # 9696-CD) is immobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated Rat IgG with an ED50 of 1.25-6.25 μg/mL.
Background: Fc gamma RIIA/CD32a
Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, cytokine release, and B cell proliferation. There are three classes of Fc gamma Rs, based on close relationships in their extracellular domains: Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. Each group may be encoded by multiple genes and exists in different isoforms depending on species and cell type (1-3). In humans, there are three Fc gamma RII genes that encode multiple transcripts that differ in their cytoplasmic tails. Only one murine Fc gamma RII gene has been identified which has two transcripts that are homologues of the human Fc gamma RIIB (4). Fc gamma RIIA is not expressed in mice. Approximately 40 kDa, Fc gamma RIIA is a type 1 transmembrane glycoprotein that is expressed on different cell types, including, neutrophils, monocytes, dendritic cells, and is the only Fc gamma R expressed on human platelets (5). The mature protein consists of an extracellular domain (ECD), a transmembrane segment, and a cytoplasmic domain. Within the ECD, rat Fc gamma RIIA shares 52% amino acid sequence identity with human Fc gamma RIIA. The Fc gamma RI/CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the Fc gamma RII/CD32 and Fc gamma RIII/CD16 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 6). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 7). The only inhibitory member in human and mouse, Fc gamma RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (7).
- Van de Winkel, J. and P. Capel (1993) Immunol. Today. 14:215.
- Raghavan, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
- Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
- Ravetch, J.V. and J.P. Kinet (1991) Annu. Rev. Immunol. 9:457.
- Arman, M. and K. Krauel (2015) J. Thromb. Haemost. 13:893.
- Takai, T. (2002) Nature Rev. Immunol. 2:580.
- Ravetch, J. and L. Lanier (2000) Science 290:84.
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