Recombinant Rat GITR/TNFRSF18 Fc Chimera Protein, CF Summary
Accession # NP_001019520
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Rat GITR/TNFRSF18 Fc Chimera is immobilized at 2 µg/mL,Recombinant Mouse GITR Ligand/TNFSF18 aa 47-173 (Catalog # 2177-GL) binds with an ED50 of 25-150 ng/mL.
GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation (1, 2). Mature rat GITR consists of a 134 amino acid (aa) extracellular domain (ECD) with three tandem TNFR cysteine-rich repeats, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain. Within the ECD, rat GITR shares 60% and 86% aa sequence identity with human and mouse GITR, respectively. Alternative splicing generates additional isoforms with an N-terminal extension or a substitution in the cytoplasmic domain. GITR is expressed on CD4+CD25+ regulatory T cells (Treg) as well as on subsets of thymocytes, lymph node cells, and splenocytes (3-5), and it is up-regulated on antigen-activated conventional CD4+ and CD8+ T cells (4-7). GITR binding by GITR Ligand/TNFSF18 costimulates the proliferation and activation of CD4+ or CD8+ conventional T cells (6-9). It also induces the proliferation of Treg (8, 10) but inhibits the ability of Treg to suppress immune responses (3, 8, 11-13). This can result in the development of autoimmunity, increased tumor cell killing by effector T cells (3, 11), and increased inflammation in arthritis, allergic asthma, and inflammatory bowel disease (10, 14). GITR is also expressed on sympathetic neurons where it enhances NGF-induced neurite outgrowth and branching (15).
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Citation for Recombinant Rat GITR/TNFRSF18 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
GITR/GITRL reverse signalling modulates the proliferation of hepatic progenitor cells by recruiting ANXA2 to phosphorylate ERK1/2 and Akt
Authors: Y He, Y Pei, K Liu, L Liu, Y Tian, H Li, M Cong, T Liu, H Ma, H You, J Jia, D Zhang, P Wang
Cell Death & Disease, 2022;13(4):297.
Sample Types: Whole Cells
Applications: Cell Culture
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