Recombinant Rat IL-1 RI Fc Chimera Protein, CF Summary
|Rat IL-1 RI
(Leu20 - Lys338)
Accession # Q02955
(Glu98 - Lys330)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 1 mg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: IL-1 RI
The type I IL-1 receptor (IL-1 RI, designated IL-1 R1 and CD121a) is one of at least nine members of the IL-1 R family within the Toll/IL-1 R (TIR) superfamily (1 - 3). IL-1 RI is an 80 kDa type I transmembrane (TM) protein that binds the pleiotropic cytokines IL-1 alpha and IL-1 beta, plus the IL-1 receptor antagonist (IL-1 Ra). Signal transduction requires complex formation with the IL-1 R accessory protein (IL-1 R AcP/IL-1 R3), another type I TM protein (1, 2). This complex recruits the adaptor protein MyD88, to initiate signaling in the NF kappa B pathway (4, 5). Rat IL-1 RI cDNA encodes a 576 amino acid (aa) protein that contains a 19 aa signal sequence, a 319 aa extracellular domain (ECD) with three C2-type Ig-like domains, a 21 aa TM domain and a 217 aa cytoplasmic region with a TIR domain. Rat IL-1 RI shares 83%, 65%, 60%, 60% and 54% aa identity with mouse, human, canine, equine and bovine IL-1 RI, respectively. Two additional splice isoforms of rat IL-1 RI have been described (6). One has an N-terminus that is extended by 14 amino acids, but appears to have equivalent function. The other lacks the TM sequence and is secreted as an ~82 kDa protein that antagonizes the effect of IL-1 beta. Thus, the role of IL-1 in inflammation is under several levels of control, including expression and activation of IL-1 alpha and IL-1 beta, expression of IL-1 RI and its accessory and adaptor proteins, and inhibitory IL-1 R isoforms and decoys (1 - 6). IL-1 RI is expressed predominantly by T cells, fibroblasts, and endothelial cells and mediates acute phase inflammatory responses including fever (1, 2, 5, 7).
- Boraschi, D. & A. Tagliabue (2006) Vitam. Horm. 74:229.
- Dinarello, C.A. (2002) Clin. Exp. Rheumatol. 20:S1.
- Hart, R.P. et al. (1993) J. Neuroimmunol. 44:49.
- Brikos, C. et al. (2007) Mol. Cell. Proteomics 6:1551.
- Gasse, P. et al. (2007) J. Clin. Invest. 117:3786.
- Yamada, M. et al. (2007) Nitric Oxide 17:98.
- Ching, S. et al. (2007) J. Neurosci. 27:10476.
Citation for Recombinant Rat IL-1 RI Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
P2X7 receptor inhibition attenuated sympathetic nerve sprouting after myocardial infarction via the NLRP3/IL-1? pathway
Authors: J Yin, Y Wang, H Hu, X Li, M Xue, W Cheng, Y Wang, X Li, N Yang, Y Shi, S Yan
J. Cell. Mol. Med., 2017;0(0):.
Sample Types: In Vivo
Applications: In Vivo
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Rat IL-1 RI Fc Chimera Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Rat IL-1 RI Fc Chimera Protein, CF and earn rewards!
Have you used Recombinant Rat IL-1 RI Fc Chimera Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image