Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines, which have important immunological functions. IL-12 is composed of two disulfide-linked subunits of 35 kDa and 40 kDa, respectively. The 35 kDa subunit (p35) is an alpha -helical protein homologous to IL-6 and G‑CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also utilized in IL-23. Mature rat p35 is a 194 amino acids (aa) protein that is secreted as a heterodimer linked to p40. It contains three potential N-linked glycosylation sites and shares 86%, and 58% aa sequence identity with mouse and human p35, respectively. Mature rat p40 contains 313 aa and can exist in multiple forms, including monomer, homodimer, heterodimer linked to p19 (forming IL-23), and heterodimer linked to p35 (forming IL-12). The expression of p40 is upregulated by substances such as LPS and CpG that activate antigen-presenting cells. Mature rat p40 shows 92% and 66% aa sequence identity to mouse and human p40, respectively. Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. The activities of IL-12 are mediated by the receptor complex composed of two type I transmembrane proteins: a 100 kDa ligand-binding subunit (IL‑12 R beta 1) and a 130 kDa signal transducing subunit (IL‑12 R beta 2). IL‑12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, and potentiates the expansion and late activation of Th1 CD4+ T cells (1 - 4).
Key Product Details
- R&D Systems Sf 21 (baculovirus)-derived Recombinant Rat IL-12 Protein (1760-RL)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Structure / Form
Applications
Product Specifications
Source
| Rat IL-12 p40 (Met23 - Ser335) Accession # NP_072133 |
6-His tag |
| Rat IL-12 p35 (Arg23 - Ser215) Accession # Q9R103 |
6-His tag |
| N-terminus | C-terminus |
Purity
Endotoxin Level
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 0.025-0.25 ng/mL.
Reviewed Applications
Read 1 review rated 5 using 1760-RL in the following applications:
Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1760-RL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 1760-RL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: IL-12
References
- Park, A.Y. and P. Scott (2001) Scand. J. Immunol. 53:529.
- Trinchieri, G. et al. (2003) Immunity 19:641.
- Brombacher, F. et al. (2003) Trends Immunol. 24:207.
- Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional IL-12 Products
Product Documents for Recombinant Rat IL-12 Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Rat IL-12 Protein
For research use only
Related Research Areas
Citations for Recombinant Rat IL-12 Protein
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Application: Immunoassay StandardVerified Customer | Posted 11/08/2017The recombinant rat IL-12 was used to build an ELISA standard curve in an assay developed using AF1760 as both the capture and detection. Il-12 present in rat serum samples was measured.
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