Recombinant Rat IL-12 Protein

Formulations:
Catalog # Availability Size / Price Qty
1760-RL-010
Product Details
Citations (2)
FAQs
Reviews (1)

Recombinant Rat IL-12 Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The ED50 for this effect is 0.025-0.25 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat IL-12 protein
Rat IL-12 p40
(Met23 - Ser335)
Accession # NP_072133
6-His tag
Rat IL-12 p35
(Arg23 - Ser215)
Accession # Q9R103
6-His tag
N-terminus C-terminus
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
36.8 kDa (p40), 22.6 kDa (p35)
SDS-PAGE
42-45 kDa and 26-28 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1760-RL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1760-RL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-12

Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines, which have important immunological functions. IL-12 is composed of two disulfide-linked subunits of 35 kDa and 40 kDa, respectively. The 35 kDa subunit (p35) is an alpha -helical protein homologous to IL-6 and G‑CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also utilized in IL-23. Mature rat p35 is a 194 amino acids (aa) protein that is secreted as a heterodimer linked to p40. It contains three potential N-linked glycosylation sites and shares 86%, and 58% aa sequence identity with mouse and human p35, respectively. Mature rat p40 contains 313 aa and can exist in multiple forms, including monomer, homodimer, heterodimer linked to p19 (forming IL-23), and heterodimer linked to p35 (forming IL-12). The expression of p40 is upregulated by substances such as LPS and CpG that activate antigen-presenting cells. Mature rat p40 shows 92% and 66% aa sequence identity to mouse and human p40, respectively. Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. The activities of IL-12 are mediated by the receptor complex composed of two type I transmembrane proteins: a 100 kDa ligand-binding subunit (IL‑12 R beta 1) and a 130 kDa signal transducing subunit (IL‑12 R beta 2). IL‑12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, and potentiates the expansion and late activation of Th1 CD4+ T cells (1 - 4).

References
  1. Park, A.Y. and P. Scott (2001) Scand. J. Immunol. 53:529.
  2. Trinchieri, G. et al. (2003) Immunity 19:641.
  3. Brombacher, F. et al. (2003) Trends Immunol. 24:207.
  4. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
Long Name
Interleukin 12
Entrez Gene IDs
3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)
Alternate Names
CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35

Citations for Recombinant Rat IL-12 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Dendritic cell-targeted CD40 DNA vaccine suppresses Th17 and ameliorates progression of experimental autoimmune glomerulonephritis
    Authors: Q Li, Q Cao, C Wang, H Nguyen, XM Wang, G Zheng, YM Wang, S Hu, SI Alexander, DCH Harris, Y Wang
    J. Leukoc. Biol., 2019;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Analytical performance of a commercial multiplex Luminex-based cytokine panel in the rat
    Authors: T Erkens, N Goeminne, A Kegels, M Byloos, P Vinken
    J Pharmacol Toxicol Methods, 2018;91(0):43-49.
    Species: Rat
    Sample Types: Serum
    Applications: Multiplex Assay

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Reviews for Recombinant Rat IL-12 Protein

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Recombinant Rat IL-12 Protein
By Anonymous on 11/08/2017
Application: Immunoassay Standard

The recombinant rat IL-12 was used to build an ELISA standard curve in an assay developed using AF1760 as both the capture and detection. Il-12 present in rat serum samples was measured.