Recombinant Rat IL-1ra/IL-1F3 Protein

  (11 citations)     
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Product Details
Citations (11)
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  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit IL-1 alpha -dependent proliferation in D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 38-150 ng/mL in the presence of 50 pg/mL of rrIL-1 alpha.
  • Source
    E. coli-derived rat IL-1ra/IL-1F3 protein
    His27-Gln178, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    17.5 kDa
Product Datasheets

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Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1545-RA
 
1545-RA/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1ra/IL-1F3

Interleukin 1 alpha (IL-1 alpha ), IL-1 beta, and IL-1 receptor antagonist (IL-1ra) are members of the IL-1 family. IL-1 alpha and IL-1 beta are agonists that bind the functional IL-1 receptor I (IL-1 RI), which then recruits the IL-1 receptor accessory protein (IL-1 R AcP/IL-1 R3) and initiates signal transduction pathways that include the NF-kappa B. JNK/AP-1, and p38 MAP kinase pathways. Both IL-1 alpha and IL-1 beta also bind the non‑signaling IL-1 RII that functions as a decoy receptor. IL-1ra is a receptor antagonist that binds both IL-1 RI and II with equal affinity as the IL-1 alpha and beta. Binding of IL-1ra to IL-1 RI does not initiate signal transduction pathways. IL-1 alpha and beta are pro‑inflammatory cytokines that play an important role in host defense. The imbalance between IL-1 ( alpha  and  beta ) and IL-1ra can influence the development of various pathologic conditions associated with chronic inflammation and tissue destruction. Pre‑clinical and clinical studies have indicated that IL-1ra may have therapeutic potential for the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia (1, 2).

Rat IL-1ra cDNA encodes a 178 amino acid (aa) residues secreted protein with a 26 aa signal peptide. Although intracellular isoforms of human IL-1ra that are transcribed from the same gene using different promotors have been described, analogous rat isoforms have not been reported. Rat IL-1ra shares 89% and 73% aa sequence homology with mouse and human IL-1ra, respectively. Secreted IL-1ra has been shown to be expressed by monocytes, macrophages, neutrophils, fibroblasts and hepatocytes (1, 2).

  • References:
    1. Dinarello, C.A. (1996) Blood 87:2095 (review).
    2. Arend, W.P. (2002) Growth Factor Reviews 23:323 (review).
  • Long Name:
    Interleukin 1 Receptor Antagonist
  • Entrez Gene IDs:
    3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)
  • Alternate Names:
    DIRA; ICIL-1RA; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms
    Authors: L Taoro-Gonz, YM Arenas, A Cabrera-Pa, V Felipo
    J Neuroinflammation, 2018;15(1):36.
    Species: Rat
    Sample Type: Whole Tissue
    Application: Bioassay
  2. A leptin-mediated central mechanism in analgesia-enhanced opioid reward in rats.
    Authors: Lim G, Kim H, McCabe M, Chou C, Wang S, Chen L, Marota J, Blood A, Breiter H, Mao J
    J Neurosci, 2014;34(29):9779-88.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  3. Brain interleukin-1beta and the intrinsic receptor antagonist control peripheral Toll-like receptor 3-mediated suppression of spontaneous activity in rats.
    Authors: Yamato M, Tamura Y, Eguchi A, Kume S, Miyashige Y, Nakano M, Watanabe Y, Kataoka Y
    PLoS ONE, 2014;9(3):e90950.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  4. Possible protective effect of membrane lipid rafts against interleukin-1beta-mediated anti-proliferative effect in INS-1 cells.
    Authors: Chentouf M, Guzman C, Hamze M, Gross R, Lajoix A, Peraldi-Roux S
    PLoS ONE, 2014;9(7):e102889.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  5. Unmasking of LPA1 receptor-mediated migration response to lysophosphatidic acid by interleukin-1beta-induced attenuation of Rho signaling pathways in rat astrocytes.
    Authors: Sato K, Horiuchi Y, Jin Y, Malchinkhuu E, Komachi M, Kondo T, Okajima F
    J. Neurochem., 2011;117(1):164-74.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  6. Reductions of acetylcholine release and nerve growth factor expression are correlated with memory impairment induced by interleukin-1beta administrations: effects of omega-3 fatty acid EPA treatment.
    Authors: Taepavarapruk P, Song C,
    J. Neurochem., 2010;112(4):1054-64.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  7. Lysophosphatidic acid signals through multiple receptors in osteoclasts to elevate cytosolic calcium concentration, evoke retraction, and promote cell survival.
    Authors: Lapierre DM, Tanabe N, Pereverzev A
    J. Biol. Chem., 2010;285(33):25792-801.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  8. Antagonism of peripheral inflammation reduces the severity of status epilepticus.
    Authors: Marchi N, Fan Q, Ghosh C, Fazio V, Bertolini F, Betto G, Batra A, Carlton E, Najm I, Granata T, Janigro D
    Neurobiol. Dis., 2009;33(2):171-81.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  9. IL-1beta is an essential mediator of the antineurogenic and anhedonic effects of stress.
    Authors: Koo JW, Duman RS
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(2):751-6.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  10. Interleukin-1 beta contributes to the upregulation of kappa opioid receptor mrna in dorsal root ganglia in response to peripheral inflammation.
    Authors: Puehler W, Rittner HL, Mousa SA, Brack A, Krause H, Stein C, Schafer M
    Neuroscience, 2006;141(2):989-98.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  11. The effects of endogenous interleukin-1 bioactivity on locus coeruleus neurons in response to bacterial and viral substances.
    Authors: Borsody MK, Weiss JM
    Brain Res., 2004;1007(1):39-56.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
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