Recombinant Rat IL-1ra/IL-1F3 Protein

Name: Recombinant Rat IL-1ra/IL-1F3 Protein
Brand: R&D Systems
SKU: 1545-RA
Price: 479 USD
Availability: InStock

R&D Systems | Catalog # 1545-RA

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Citations (16)

Product Documents

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems E. coli-derived Recombinant Rat IL-1ra/IL-1F3 Protein (1545-RA)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P25086.1

Applications

Bioactivity

View all IL-1ra/IL-1F3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived rat IL-1ra/IL-1F3 protein
His27-Gln178, with an N-terminal Met

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

17.5 kDa

Activity

Measured by its ability to inhibit IL-1 alpha -dependent proliferation in D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
The ED₅₀ for this effect is 38-150 ng/mL in the presence of 50 pg/mL of rrIL-1 alpha.

Formulation, Preparation, and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1545-RA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Carrier Free: 1545-RA/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-1ra/IL-1F3

Interleukin 1 alpha (IL-1 alpha ), IL-1 beta, and IL-1 receptor antagonist (IL-1ra) are members of the IL-1 family. IL-1 alpha and IL-1 beta are agonists that bind the functional IL-1 receptor I (IL-1 RI), which then recruits the IL-1 receptor accessory protein (IL-1 R AcP/IL-1 R3) and initiates signal transduction pathways that include the NF-kappa B. JNK/AP-1, and p38 MAP kinase pathways. Both IL-1 alpha and IL-1 beta also bind the non‑signaling IL-1 RII that functions as a decoy receptor. IL-1ra is a receptor antagonist that binds both IL-1 RI and II with equal affinity as the IL-1 alpha and beta. Binding of IL-1ra to IL-1 RI does not initiate signal transduction pathways. IL-1 alpha and beta are pro‑inflammatory cytokines that play an important role in host defense. The imbalance between IL-1 ( alpha and beta ) and IL-1ra can influence the development of various pathologic conditions associated with chronic inflammation and tissue destruction. Pre‑clinical and clinical studies have indicated that IL-1ra may have therapeutic potential for the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia (1, 2).

Rat IL-1ra cDNA encodes a 178 amino acid (aa) residues secreted protein with a 26 aa signal peptide. Although intracellular isoforms of human IL-1ra that are transcribed from the same gene using different promotors have been described, analogous rat isoforms have not been reported. Rat IL-1ra shares 89% and 73% aa sequence homology with mouse and human IL-1ra, respectively. Secreted IL-1ra has been shown to be expressed by monocytes, macrophages, neutrophils, fibroblasts and hepatocytes (1, 2).