Recombinant Rat Jagged 1 His Tagged Protein, CF Summary
Ser32-Asp1067, with substitutions GGARN56-60AE and DR63-64TLVRPY and a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||
Immobilized Recombinant Rat Jagged 1 (Catalog # 9907-JG) enhances Recombinant Human/Mouse/Rat BMP-2 Recombinant Human BMP-2 (Catalog # 355-BM) alkaline phosphatase inducing activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4-2.4 µg/mL.
2 μg/lane of Recombinant Rat Jagged 1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 134-160 kDa.
Background: Jagged 1
Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins (1). Jagged 1 is synthesized as a precursor protein that contains a 33 amino acid (aa) signal sequence, a 1034 aa extracellular region, a 26 aa transmembrane (TM) segment and a 126 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats (2). Rat Jagged 1 shows 98% and 99% aa identity to human and mouse Jagged 1 extracellular domains respectively. Relative to the extracellular region of rat Jagged 2, the aa identity is 58%. The extracellular region of Rat Jagged 1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors (3). Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature (4). For instance, soluble non-transmembrane forms of Jagged1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis (5). Soluble Jagged 1 is also capable of maintaining the survival and enhancing the expansion of human stem cells that are capable of reconstituting hematopoietic lineages in vivo (6). Furthermore, Jagged 1 is implicated in human disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged 1 (7). Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling (8).
- Ascano, J. M. et al. (2003) J. Biol. Chem. 278:8771
- Lindsell, C.E. et al. (1995) Cell 80:909.
- Shimizu, K. et al. (1999) J. Biol. Chem. 274:32961.
- Lewis, J. (1998) Stem Cell & Dev. Biol. 9:583.
- Small, D. et al. (2001) J. Biol. Chem. 276:32022.
- Karanu, F. et al. (2000) J. Exp. Med. 192:1365.
- Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
- Hicks, C. et al. (2002) J Neurosci. Res. 68:655.
Citation for Recombinant Rat Jagged 1 His Tagged Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2
Authors: S Kakuda, RK Lopilato, A Ito, RS Haltiwange
J. Biol. Chem., 2020;0(0):.
Sample Types: Whole Cells
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