Recombinant Rat Neuroligin 1/NLGN1 Protein, CF Summary
Met1-Leu676, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Neuroligin 1/NLGN1
Neuroligin 1 (NLGN1 or NL1) is one of several type I transmembrane Neuroligin proteins that play an important role in synaptic development (1). Mature rat Neuroligin 1 is an N- and O-glycosylated 120 kDa molecule that consists of a 652 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 125 aa cytoplasmic tail (2). Insertion of 20 aa at splice site A and/or 9 aa at splice site B within the ECD results in NLGN1A, NLGN1B, or NLGN1AB isoforms while the NLGN1(-) isoform has neither insertion (3). This recombinant protein (NLGN1B) contains the insert at splice site B but not the insert at splice site A. Within the ECD, rat NLGN1B shares 99% aa sequence identity with comparable regions of human and mouse Neuroligin 1. Neuroligin 1 expression is restricted to postsynaptic nerve terminal membranes where it associates into dimers and tetramers (5 - 7). NLGN1B and NLGN1AB are enriched at excitatory (glutamatergic) contacts, whereas NLGN1A is enriched at inhibitory (GABAergic) contacts and NLGN1(-) is expressed at both (3). The interaction of Neuroligin 1 with presynaptic membrane Neurexins promotes the maturation of presynaptic as well as postsynaptic structures (3, 4, 8 - 11). Neuroligin 1 isoforms are selective in which Neurexin isoforms they bind: NLGN1B and NLGN1AB preferentially interact with Neurexin 1 beta lacking the splice site 4 insert, while NLGN1A and NLGN1(-) bind Neurexin 1 alpha and Neurexin 1 beta irrespective of the SS4 insert (12). Neurexin 1 beta (-SS4) additionally binds to LRRTM2 to trigger a second mechanism that promotes excitatory synapse development (13, 14). Neuroligin 1 mediated synaptogenesis is also enhanced by its direct binding to Thrombospondin 1 (15).
- Sudhof, T.C. (2008) Nature 455:903.
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- Chih, B. et al. (2006) Neuron 51:171.
- Dean, C. et al. (2003) Nat. Neurosci. 7:708.
- Comoletti, D. et al. (2006) Biochemistry 45:12816.
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- Wittenmayer, N. et al. (2009) Proc. Natl. Acad. Sci. 106:13564.
- Jung, S.-Y. et al. (2010) Proc. Natl. Acad. Sci. 107:4710.
- Chubykin, A.A. et al. (2007) Neuron 54:919.
- Chih, B. et al. (2005) Science 307:1324.
- Boucard, A.A. et al. (2005) Neuron 48:229.
- Siddiqui, T.J. et al. (2010) J. Neurosci. 30:7495.
- Ko, J. et al. (2009) Neuron 64:791.
- Xu, J. et al. (2010) Nat. Neurosci. 13:22.
Citations for Recombinant Rat Neuroligin 1/NLGN1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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The soluble neurexin-1&beta ectodomain causes calcium influx and augments dendritic outgrowth and synaptic transmission
Authors: KDB Wierda, TL Toft-Berte, CR Gøtzsche, E Pedersen, I Korshunova, J Nielsen, ML Bang, AB Kønig, S Owczarek, MD Gjørlund, M Schupp, E Bock, JB Sørensen
Sci Rep, 2020;10(1):18041.
Sample Types: Whole Cells
Applications: Surface Plasmon Resonance
Interaction of A? Oligomers with Neurexin 2? and Neuroligin 1 Mediates Synapse Damage and Memory Loss in Mice
Authors: J Brito-More, MV Lourenco, MM Oliveira, JF Vital, FC Ribeiro, JH Ledo, LP Diniz, MH Magdesian, HM Melo, F Barros-Ara, JM de Souza, SV Alves-Leon, FC Gomes, JR Clarke, CP Figueiredo, FG De Felice, ST Ferreira
J. Biol. Chem, 2017;0(0):.
Sample Types: Protein
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