Recombinant Rat PD-L1/B7-H1 Fc Chimera Protein, CF

R&D Systems | Catalog # 9835-B7

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Key Product Details

  • R&D Systems NS0-derived Recombinant Rat PD-L1/B7-H1 Fc Chimera Protein (9835-B7)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_001178883

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all PD-L1/B7-H1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat PD-L1/B7-H1 protein
Rat B7-H1
(Phe19-Thr238)
Accession # NP_001178883		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe19

Predicted Molecular Mass

52 kDa 

SDS-PAGE

74-84 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Rat PD-1 Fc Chimera (Catalog # 9814-PD) at 1 µg/mL (100 µL/well) can bind Recombinant Rat PD‑L1/B7-H1 Fc Chimera with an ED50 of 0.09-0.54 μg/mL.

Scientific Data Images for Recombinant Rat PD-L1/B7-H1 Fc Chimera Protein, CF

Recombinant Rat PD-L1/B7-H1 Fc Chimera Protein Binding Activity

Recombinant Rat PD-L1/B7-H1 Fc Chimera Protein Binding Activity

When Recombinant Rat PD-1 Fc Chimera (Catalog # 9814-PD) is coated at 1 µg/mL, Recombinant Rat PD-L1/B7-H1 Fc Chimera (Catalog # 9835-B7) binds with an ED50 of 0.09-0.54 µg/mL.

Formulation, Preparation, and Storage

9835-B7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 52 kDa transmembrane glycoprotein in the B7 family of immune-regulatory molecules (1). Mature rat B7-H1 consists of a 220 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (2). Within the ECD, rat B7-H1 shares 74% and 86% aa sequence identity with human and mouse, respectively. B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (3-6), keratinocytes (7, 8), enothelial and intestinal epithelial cells (8-10), as well as a variety of carcinomas and melanoma (10, 11). B7-H1 binds to T cell B7-1/CD80 and PD-1 (6, 7, 11-14). It suppresses T cell activation and proliferation (4, 7, 13, 15) and induces the apoptosis of activated T cells (10). It plays a role in the development of immune tolerance by promoting T cell anergy (6, 13) and enhancing regulatory T cell development (15). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (4, 9) and inhibits the development of Th17 cells (15). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (5, 8, 11, 14).

References

  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Dong, H. et al. (1999) Nat. Med. 5:1365.
  3. Tamura, H. et al. (2001) Blood 97:1809.
  4. Chen, L. et al. (2007) J. Immunol. 178:6634.
  5. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
  6. Tsushima, F. et al. (2007) Blood 110:180.
  7. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
  8. Cao, Y. et al. (2010) Cancer Res. 71:1235.
  9. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
  10. Dong, H. et al. (2002) Nat. Med. 8:793.
  11. Azuma, T. et al. (2008) Blood 111:3635.
  12. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
  13. Park, J.-J. et al. (2010) Blood 116:1291.
  14. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
  15. Herold, M. et al. (2015) J. Immunol. 195:3584.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

UniProt

NP_001178883

Additional PD-L1/B7-H1 Products

Product Documents for Recombinant Rat PD-L1/B7-H1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Rat PD-L1/B7-H1 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Rat PD-L1/B7-H1 Fc Chimera Protein, CF

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