Recombinant Rat PD-L2/B7-DC Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9994-PL-100
Recombinant Rat PD-L2/B7-DC Fc Chimera Protein, CF Binding Activity
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Recombinant Rat PD-L2/B7-DC Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat PD‑L2/B7‑DC Fc Chimera is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Rat PD-1 Fc Chimera (Catalog # 9814-PD) that produces 50% optimal binding response is 0.01-0.08 μg/mL
Source
Mouse myeloma cell line, NS0-derived rat PD-L2/B7-DC protein
Rat PD-L2
(Leu20-Arg219)
Accession # NP_001101052.2
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Leu20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
49 kDa
SDS-PAGE
62-73 kDa, reducing conditions

Product Datasheets

9994-PL

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and EDTA with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Recombinant Rat PD-L2/B7-DC Fc Chimera Protein, CF Binding Activity View Larger

When Recombinant Rat PD‑L2/B7‑DC Fc Chimera (Catalog # 9994-PL) is coated at 1 μg/mL (100 μL/well), Recombinant Rat PD-1 Fc Chimera (Catalog # 9814-PD) binds with an ED50  of 0.01-0.08 μg/mL.

SDS-PAGE Recombinant Rat PD-L2/B7-DC Fc Chimera Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Rat PD‑L2/B7‑DC was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 62-73 kDa and 120-150 kDa, respectively.

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Background: PD-L2/B7-DC

Programmed Death Ligand 2 (PD-L2), also known as Butyrophilin B7-DC, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). In humans, the mature PD-L2 consists of a 201 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2, 3). Within the ECD, rat PD-L2 shares 71% and 95% aa sequence identity with human and mouse PD-L2, respectively. PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3-5). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (6-10). PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).

References
  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
  3. Tseng, S.Y. et al. (2001) J. Exp. Med. 193:839.
  4. Messal, N. et al. (2011) Mol. Immunol. 48:2214.
  5. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
  6. Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
  7. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
  8. Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
  9. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
  10. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
  11. Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
  12. Shin, T. et al. (2003) J. Exp. Med. 198:31.
  13. Xiao, Y. et al. (2014) J. Exp. Med. 211:943.
Long Name
Programmed Death-1 Ligand-2
Entrez Gene IDs
80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)
Alternate Names
B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; Butyrophilin-like Protein; CD273 antigen; CD273; CD273PD-1 ligand 2; MGC142240; PD-1-ligand 2; PDCD1L2MGC142238; PDCD1LG2; PDL2; PD-L2; PDL2B7DC; PD-L2PDCD1 ligand 2; programmed cell death 1 ligand 2; Programmed death ligand 2

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