Recombinant SARS-CoV Spike RBD His-tag Protein, CF

R&D Systems | Catalog # 10558-CV

Mammalian CHO Cell Expressed
R&D Systems
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Key Product Details

  • R&D Systems CHO-derived Recombinant SARS-CoV Spike RBD His-tag Protein (10558-CV)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_828851.1

Applications

Bioactivity
View all Spike RBD Proteins and Enzymes »

Why choose R&D Systems Recombinant SARS-CoV Spike RBD His-tag Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

Interested in other Coronavirus proteins including SARS-CoV-2 Spike proteins? View Products

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived sars-cov Spike RBD protein
Arg306-Phe527,with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg306

Predicted Molecular Mass

26 kDa

SDS-PAGE

32-40 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA with Recombinant Human ACE-2 His-tag (Catalog # 933-ZN).

Reviewed Applications

Read 1 review rated 5 using 10558-CV in the following applications:

Scientific Data Images for Recombinant SARS-CoV Spike RBD His-tag Protein, CF

Recombinant SARS-CoV Spike RBD His-tag Protein Binding Activity

Recombinant SARS-CoV Spike RBD His-tag Protein Binding Activity

Recombinant SARS-CoV Spike RBD His-tag (Catalog # 10558-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA.
Recombinant SARS-CoV Spike RBD His-tag Protein SDS-PAGE

Recombinant SARS-CoV Spike RBD His-tag Protein SDS-PAGE

2 μg/lane of Recombinant SARS-CoV Spike RBD His-tag Protein (Catalog # 10558-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-40 kDa.

Formulation, Preparation, and Storage

10558-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Spike RBD

SARS-CoV was discovered in association with cases of severe acute respiratory syndrome (SARS) that infected more than 8,000 persons with over 900 fatalities worldwide in 2002-2003 (1). It belongs to a family of viruses known as coronaviruses that also include MERS and SARS-Cov2 that causes the global pandemic coronavirus disease 2019 (Covid-19). Coronavirus is commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). SARS-CoV S Protein is a type-I trimerized membrane glycoprotein that mediates membrane fusion and viral entry. As with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (2-4). A metallopeptidase, angiotensin-converting enzyme 2 (ACE-2), has been identified as a functional receptor for SARS-CoV through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit (5, 6). Based on amino acid (aa) sequence homology, the RBD domain of SARS-Cov shares 73% and 24% homology with RBD domain of SARS-CoV2 and MERS, respectively. Before binding to the ACE-2 receptor, structural analysis of the S1 trimer shows that only one of the three RBD domains in the trimeric structure is in the "up" conformation. This is an unstable and transient state that passes between trimeric subunits but is nevertheless an exposed state to be targeted for neutralizing antibody therapy (7). Antibodies to S protein especially the RBD region of SARS-CoV have been shown to inhibit interaction with the ACE-2 receptor, confirming RBD as an attractive target for vaccinations or antiviral therapy (8).

References

  1. Rota, P.A. et al. (2003) Science 300:1394.
  2. Bosch, B.J. et al. (2003). J. Virol. 77:8801.
  3. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:5871.
  4. Millet, J.K. and G. R. Whittaker (2015) Virus Res. 202:120.
  5. Li, W. et al. (2003) Nature 426:450.
  6. Wong, S.K. et al. (2004) J. Biol. Chem. 279:3197.
  7. Ortega, J.T. et al. (2020) EXCLI J. 19:410.
  8. Du, L. el al. (2009) Nat. Rev. Microbiol. 7:226.

Long Name

Spike Receptor Binding Domain

Entrez Gene IDs

3200426 (HCoV-HKU1); 14254594 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

S

UniProt

NP_828851.1

Additional Spike RBD Products

Product Documents for Recombinant SARS-CoV Spike RBD His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant SARS-CoV Spike RBD His-tag Protein, CF

For research use only

Related Research Areas

Infectious Diseases

Customer Reviews for Recombinant SARS-CoV Spike RBD His-tag Protein, CF (1)

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