Recombinant Tetanus Neurotoxin Type Light Chain Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Supplemental Products

Recombinant Tetanus Neurotoxin Type Light Chain Protein, CF Summary

Product Specifications

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by the cleavage of the substrate GFPuv/SNAP/VAMP in a gel-shift assay. >50% of 1 μg substrate is cleaved by 40 ng, as measured under the described conditions.
E. coli-derived c. tetani TeNT Light Chain protein
Pro2-Gly430, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
50 kDa
43-50 kDa, reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Tween® 20.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

  • Assay Buffer: 50 mM HEPES, 0.05% (v/v) Tween® 20, pH 7.5
  • Recombinant C. tetani TeNT Light Chain (rTeNT-LC) (Catalog # 6535-ZN)
  • Substrate: Recombinant GFP/SNAP25B/VAMP-2 (Catalog # 7375-SV)
  • SDS-PAGE and silver stain reagents or equivalent or Western blot with appropriate antibodies
  1. Dilute Substrate to 100 µg/mL in Assay Buffer.
  2. Dilute rTeNT-LC to 4 µg/mL in Assay Buffer.
  3. Combine equal volumes of diluted Substrate with diluted rTeNT-LC. Prepare two controls by combining equal volumes of diluted Substrate with Assay Buffer.
  4. Incubate reaction vials at 37 °C for 1 hour.  Incubate one control at 37 °C and the other at -20 °C for 1 hour.
  5. After incubation, combine reaction mixtures and controls with reducing SDS-PAGE sample buffer at a 1:1 (reaction mixture:sample buffer) ratio (v/v) to stop reactions.
  6. Analyze the cleavage products by SDS-PAGE (Load 40 µL of the mixture from step 5 per lane, 1 µg Substrate per lane) followed by silver staining and/or Western blot.
  • rTeNT-LC:  40 ng
  • Substrate:  1 µg
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: TeNT Light Chain

Tetanus toxin (TeNT) is a potent neurotoxin produced by Clostridium tetani. TeNT is similar in structure and function to the botulinum toxins produced by other Clostridium species (1, 2). TeNT is among the most toxic protein toxins known for humans. TeNT is synthesized as an inactive single chain precursor that undergoes proteolytic cleavage to create light and heavy chains that are linked by a disulfide bond. The 50 kDa light chain contains a metalloprotease domain whereas the 100 kDa heavy chain contains a receptor binding domain and a domain required for translocation across the cell membrane (3). Once inside a neuronal cell the zinc metalloprotease domain is able to cleave synaptobrevin to cause motor neuron disinhibition, resulting in the spastic paralysis characteristic of tetanus (4). The E. coli expressed recombinant TeNT light chain is an active protease. In the absence of heavy chains, however, it lacks toxicity because it cannot enter into host cells.

  1. Eisel, U. et al. (1986) EMBO J. 5:2495.
  2. Montecucco, C. and S. Giampietro (1993) Trends Biochem. Sci. 18:324.
  3. Schiavo, G. et al. (1992) Nature 359:832.
  4. Schiavo, G. et al. (1992) EMBO J. 11:3577.
Long Name
Tetanus Neurotoxin Type Light Chain
Entrez Gene IDs
1061100 (C. tetani)
Alternate Names
TeNT Light Chain; TeNT-LC; Tentoxylysin; Tetanus toxin chain H; Tetanus toxin chain L; Tetanus toxin heavy chain; Tetanus toxin light chain; tetX

Product Specific Notices

Coomassie is a registered trademark of Imperial Chemical Industries Ltd. Tween is a registered trademark of ICI Americas.


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Recombinant Enzymes

Reviews for Recombinant Tetanus Neurotoxin Type Light Chain Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Tetanus Neurotoxin Type Light Chain Protein, CF and earn rewards!

Have you used Recombinant Tetanus Neurotoxin Type Light Chain Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review