Reelin Antibody (CL5324) - BSA Free
Novus Biologicals | Catalog # NBP2-61414
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL5324
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P78509, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Reelin Antibody (CL5324) - BSA Free
Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414]
Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of rat cerebral cortex shows immunoreactivity in the neuropil of layer 1, as well as cytoplasmic positivity in a subset of neurons in various layers.Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414]
Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in a subset of neurons in layer 1.Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414]
Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of human tonsil shows no positivity in lymphoid cells as expected (negative control).Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414]
Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of mouse cerebral cortex shows positivity in the neuropil of layer 1, as well as cytoplasmic positivity in a subset of neurons in various layers.Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414]
Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of mouse cerebral cortex shows immunoreactivity in the neuropil of layer 1, as well as cytoplasmic positivity in a subset of neurons in various layers.Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414]
Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of mouse embryo E11 shows strong positivity in cells in the outer layer of developing cortical plate.Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414]
Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of rat cerebral cortex shows immunoreactivity in the neuropil of layer 1, as well as cytoplasmic positivity in a subset of neurons in various layers.Applications for Reelin Antibody (CL5324) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Reelin
Alternate Names
Reeler, RELN, RL
Gene Symbol
RELN
Additional Reelin Products
Product Documents for Reelin Antibody (CL5324) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Reelin Antibody (CL5324) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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