Regulatory protein GAL4 Antibody (5C8-5) - Activation Domain - BSA Free

Novus Biologicals | Catalog # NBP2-66603

Novus Biologicals
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Key Product Details

Species Reactivity

Yeast

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5C8-5

Format

BSA Free
View all Regulatory protein GAL4 Primary Antibodies »

Product Specifications

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with full length Regulatory protein GAL4 expressed in E. Coli and mouse myeloma cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

Immunoprecipitation

1:10 - 1:500

Western Blot

5-10 ug/ml
Application Notes
Recognizes Regulatory protein GAL4 derivatives containing the C-terminal activation domain. Optimal concentration should be evaluated by serial dilutions..

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Regulatory protein GAL4

GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

Alternate Names

GAL4;GAL81;YPL248C

Additional Regulatory protein GAL4 Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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