Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)

Name: Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-62162

Novus Biologicals | Catalog # NBP2-62162

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Product Documents

Discontinued Product

NBP2-62162 has been discontinued. View all RelA/NFkB p65 products.

Product Specifications
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Human, mouse, and rat cell lysates (50 uL)

Sensitivity

N/A

View all RelA/NFkB p65 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human, Mouse, Rat

Description

The NF?B p65 ELISA kit allows the accurate measurement of total and phosphorylated human NFkB p65 in cell lysates. This ELISA uses the traditional sandwich ELISA format, but with a major difference: both the sample and the assay reagents can be added to the assay microplate at the same time. Since the antibodies are not precoated in the wells, several different targets can be analyzed simultaneously in the same plate in different wells. With this kit a mixture of capture and detection antibodies against either phosphorylated or total NFkB can be added to the sample lysate, thus enabling their detection with a simple 1-hour protocol.

Kit Contents for Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)

10X wash buffer
capture antibody
cell lysis buffer
detection reagent
detector antibody
Microtiter plate
plate sealer
positive control lysate
stop solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: RelA/NFkB p65

Stimulation of the canonical NFkB pathway leads to the phosphorylation of and subsequent degradation of IkB; phosphorylation of the latter is controlled by a specific set of proteins referred to as the I Kappa Kinases (IKK), which include IKKa and IKKb. Under normal conditions, IkB acts as a key regulatory molecule in that it sequesters p65 or Rel A within the cytoplasm; this blocks NFkB dependent transcription. Upon phosphorylation of IkB by IKKs, p65 is released and free to associate with the p50 subunit; p65 acts as a transactivator whereas p50 possesses DNA binding activity. Complex formation leads to the subsequent translocation of the NFkB complex into the nucleus and the concomitant up-regulation of NFkB dependent genes. Disruption of this signaling nexus has been documented in a number of human pathological conditions including inflammation, infection and cancer.

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Alternate Names

NFkB p65, NFKB3, p65RelA

Gene Symbol

RELA

Additional RelA/NFkB p65 Products

Product Documents for Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)

Download Product Datasheets

Download NBP2-62162 ELISA Kit Manual

Certificate of Analysis

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Product Specific Notices for Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.