RENT1/UPF1/hUPF1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-370

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all RENT1/UPF1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1075 and the C-terminus (residue 1118) of human Regulator of Nonsense Transcripts 1 (up-frameshift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RENT1/UPF1/hUPF1 Antibody - BSA Free

Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Detection of human RENT1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, RKO, MCF-7, Jurkat, TMCK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Goat anti-RENT1 antibody NB100-370 used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Whole cell lysate (30 ug) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody used at 1 ug/ml.
Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RENT1 antibody (lot For blotting immunoprecipitated RENT1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-RENT1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody [NB100-370]

Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody [NB100-370]

Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Detection of human RENT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Goat anti-RENT1 antibody NB100-370 (NB100-370 lot 4) used for IP at 6 ug per reaction. RENT1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-370-3). For blotting immunoprecipitated RENT1, NB100-370 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for RENT1/UPF1/hUPF1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 -10 ug/mg lysate
Application Notes
By Western blot, this antibody recognizes a band at ~145 kDa, representing human RENT1. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RENT1/UPF1

RENT1 (regulator of nonsense transcripts 1) is a group 1 RNA helicase, RNA-binding protein whose RNA-binding activity is modulated by ATP. It enters the nucleus where it influences early events in mRNA biogenesis. It functions in NMD (non-sense-mediated mRNA decay) in mammalian cells, ridding cells of aberrant mRNAs containing premature termination codons.

Long Name

Regulator of Nonsense Transcripts 1

Alternate Names

NORF1, UPF1

Entrez Gene IDs

5976 (Human)

Gene Symbol

UPF1

UniProt

NP_002902.2 (Human)

Additional RENT1/UPF1 Products

Product Documents for RENT1/UPF1/hUPF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RENT1/UPF1/hUPF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RENT1/UPF1/hUPF1 Antibody - BSA Free

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