Rev-erb A alpha/NR1D1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84931

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Amphibian

Cited:

Mouse

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVA

Reactivity Notes

Amphibian reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rev-erb A alpha/NR1D1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody [NBP1-84931]

Immunocytochemistry/ Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody [NBP1-84931]

Immunocytochemistry/Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody [NBP1-84931] - Staining of human cell line HaCaT shows localization to nuclear bodies. Antibody staining is shown in green.

Applications for Rev-erb A alpha/NR1D1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry-Frozen

Validated from a verified customer review.
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-84931 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rev-erb A alpha/NR1D1

NR1D1 functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine

Alternate Names

Ear-1, NR1D1, Reverb A alpha, THRA1, THRAL

Gene Symbol

NR1D1

Additional Rev-erb A alpha/NR1D1 Products

Product Documents for Rev-erb A alpha/NR1D1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rev-erb A alpha/NR1D1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rev-erb A alpha/NR1D1 Antibody - BSA Free

Customer Reviews for Rev-erb A alpha/NR1D1 Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images


Showing  1 - 2 of 2 reviews Showing All
Filter By:
  • Rev-erb A alpha/NR1D1 Antibody
    Name: Deborah Lutterschmidt
    Application: Immunohistochemistry-Frozen
    Sample Tested: Brain
    Species: hyla cinerea
    Verified Customer | Posted 10/23/2023
    IHC fixed frozen with DAB staining in green tree frog brain, 10X. 1:1000 dilution
    Rev-erb A alpha/NR1D1 Antibody - BSA Free NBP1-84931
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Rev-erb A alpha/NR1D1 Antibody
    Name: Liyong Zhang
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: human primary hepatocytes
    Species: Human
    Verified Customer | Posted 12/15/2017
    immunofluorescent staining of iNOS (green) and NRIDI (white)
    Name: Anti-NRIDI antibody (NBP1-84931) Catalog #: Anti-NRIDI antibody (NBP1-84931) Lot Number: Anti-NRIDI antibody (NBP1-84931, Lot # A78474) Fixation: 2% paraformaldehyde at room temperature for 15 min Permeability: 0.1% Triton X-100 in TBS for 5-10 minutes Blocking: 2% BSA in PBS for 1 hr at room temperature Primary antibody: iNOS, 1/200; NRIDI1/200, overnight at 4°C Secondary antibody: Goat anti-Rabbit IgG H&L at 1:500 in PBS+0.5% BSA (PBB)
    Rev-erb A alpha/NR1D1 Antibody - BSA Free NBP1-84931

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Protocols

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