Rev-erb A alpha/NR1D1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-84931
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Key Product Details
Species Reactivity
Validated:
Human, Amphibian
Cited:
Mouse
Predicted:
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVA
Reactivity Notes
Amphibian reactivity reported from a verified customer review.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Rev-erb A alpha/NR1D1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody [NBP1-84931]
Immunocytochemistry/Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody [NBP1-84931] - Staining of human cell line HaCaT shows localization to nuclear bodies. Antibody staining is shown in green.Applications for Rev-erb A alpha/NR1D1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry-Frozen
Validated from a verified customer review.
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Reviewed Applications
Read 2 reviews rated 4 using NBP1-84931 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rev-erb A alpha/NR1D1
Alternate Names
Ear-1, NR1D1, Reverb A alpha, THRA1, THRAL
Gene Symbol
NR1D1
Additional Rev-erb A alpha/NR1D1 Products
Product Documents for Rev-erb A alpha/NR1D1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Rev-erb A alpha/NR1D1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Rev-erb A alpha/NR1D1 Antibody - BSA Free
Customer Reviews for Rev-erb A alpha/NR1D1 Antibody - BSA Free (2)
4 out of 5
2 Customer Ratings
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Customer Images
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2 reviews
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Application: Immunohistochemistry-FrozenSample Tested: BrainSpecies: hyla cinereaVerified Customer | Posted 10/23/2023IHC fixed frozen with DAB staining in green tree frog brain, 10X. 1:1000 dilutionBio-Techne ResponseThis review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: human primary hepatocytesSpecies: HumanVerified Customer | Posted 12/15/2017immunofluorescent staining of iNOS (green) and NRIDI (white)Name: Anti-NRIDI antibody (NBP1-84931) Catalog #: Anti-NRIDI antibody (NBP1-84931) Lot Number: Anti-NRIDI antibody (NBP1-84931, Lot # A78474) Fixation: 2% paraformaldehyde at room temperature for 15 min Permeability: 0.1% Triton X-100 in TBS for 5-10 minutes Blocking: 2% BSA in PBS for 1 hr at room temperature Primary antibody: iNOS, 1/200; NRIDI1/200, overnight at 4°C Secondary antibody: Goat anti-Rabbit IgG H&L at 1:500 in PBS+0.5% BSA (PBB)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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