RFC1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RFC1 Antibody [NBP2-54960]

Immunocytochemistry/Immunofluorescence: RFC1 Antibody [NBP2-54960] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.

Western Blot: RFC1 Antibody - BSA Free [NBP2-54960] -

DEK- or NUMA1-depleted cells showed abnormal PCNA accumulation.A–C U2OS cells were transfected with DEK or NUMA1 siRNAs. 48 h after transfection, cells were irradiated through a 5 um micropore filter with 100 J/m2 of UV-C, fixed at the indicated time points, and immunostained for PCNA and CPD. A Representative images of PCNA co-localization with CPDs. Scale bar, 20 μm. B Quantification of PCNA co-localization with CPDs (%). Each colored asterisk represents the statistical analysis in comparison to si-Con. Quantification of PCNA signal intensity at CPD foci. A.U., arbitrary unit. B, C S phase cells with S phase-specific PCNA foci staining pattern were excluded from the analysis. Error bars represent SEM (n = 3). D–G U2OS cells were transfected with siRNAs as indicated. 48 h after transfection, cells were irradiated through a 5 um micropore filter with 100 J/m2 of UV-C and subjected to immunostaining D–F or lysed for Western blot G. D Representative images. Scale bar, 20 μm. E, F Quantification of PCNA signal intensity at CPD foci (A. U.). S phase cells with S phase-specific PCNA foci staining pattern were excluded from the analysis. Images from the 30 min E and 4 h F time points were analyzed using the same parameters, and the graphs are shown separately for each time point. Error bars represent SEM (n = 3). Statistical analysis: one-way ANOVA B, C, E, F, ***P < 0.005, **P < 0.01, *P < 0.05, and ns not significant. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41420-025-02823-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.