RFWD3 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-68207

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human ring finger and WD repeat domain 3 using the numbering given in entry NP_060594.3 (GeneID 55159).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFWD3 Antibody - BSA Free

Immunoprecipitation: RFWD3 Antibody [NB100-68207] -

Detection of Human RFWD3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RFWD3 antibody NB100-68207 used for IP at 3 mcg/mg lysate. RFWD3 was also immunoprecipitated by rabbit anti-RFWD3 antibody NB100-68206, which recognizes an uptream epitope. For blotting immunoprecipitated RFWD3, NB100-68208 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for RFWD3 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500
Application Notes
This antibody is not recommended for Western Blot analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RFWD3

RFWD3 (ring finger and WD repeat domain 3) has been identified as a protein that is phosphorylated in response to DNA damage. The exact function of RFWD3 (ring finger and WD repeat domain 3) has not been characterized. Structurally, RFWD3 possesses a RING-type zinc finger and 3 WD repeats.

Alternate Names

EC 6.3.2.-, FLJ10520, ring finger and WD repeat domain 3, RING finger and WD repeat domain-containing protein 3, RING finger protein 201, RNF201E3 ubiquitin-protein ligase RFWD3

Entrez Gene IDs

55159 (Human)

Gene Symbol

RFWD3

Additional RFWD3 Products

Product Documents for RFWD3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RFWD3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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