RFX1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13223

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RFX1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: SPLQLTNIQVPQQALPTQRLVVQSAAPGSKGGQVSLTVHGTQQVHSPPEQSPVQANSSSSKTAGAPTGTVPQQLQVHGVQQSVPVTQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFX1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RFX1 Antibody [NBP2-13223]

Immunocytochemistry/ Immunofluorescence: RFX1 Antibody [NBP2-13223]

Immunocytochemistry/Immunofluorescence: RFX1 Antibody [NBP2-13223] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: RFX1 Antibody [NBP2-13223]

Immunohistochemistry-Paraffin: RFX1 Antibody [NBP2-13223]

Immunohistochemistry-Paraffin: RFX1 Antibody [NBP2-13223] - Staining of human cerebral cortex shows nuclear positivity in neurons.
RFX1 Antibody - BSA Free Immunohistochemistry: RFX1 Antibody - BSA Free [NBP2-13223]

Immunohistochemistry: RFX1 Antibody - BSA Free [NBP2-13223]

Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
RFX1 Antibody - BSA Free Immunohistochemistry: RFX1 Antibody - BSA Free [NBP2-13223]

Immunohistochemistry: RFX1 Antibody - BSA Free [NBP2-13223]

Staining of human lymph node shows no positivity in non-germinal center cells as expected.
RFX1 Antibody - BSA Free Immunohistochemistry: RFX1 Antibody - BSA Free [NBP2-13223]

Immunohistochemistry: RFX1 Antibody - BSA Free [NBP2-13223]

Staining of human cerebral cortex shows strong nuclear positivity in neurons.
RFX1 Antibody - BSA Free Immunohistochemistry: RFX1 Antibody - BSA Free [NBP2-13223]

Immunohistochemistry: RFX1 Antibody - BSA Free [NBP2-13223]

Staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.

Applications for RFX1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RFX1

RFX1, also known as MHC class II regulatory factor RFX1, is a 105 kDa 979 amino acid protein found in the nucleus. The gene plays a significant role in the gene expression of MHC class II. Current research on RFX1 is being conducted in relation to hepatitis, leukemia, neuroblastoma, sickle cell anemia and adenocarcinoma. RFX1 has been linked to the methlation, immune response, spermatogenesis and localization pathways where it interacts with TADA3, SMAD1, HDAC1, ADD1 and SMAD9.

Alternate Names

EFC, EF-C, Enhancer factor C, MHC class II regulatory factor RFX, MHC class II regulatory factor RFX1, Regulatory factor X 1, regulatory factor X, 1 (influences HLA class II expression), RFX, trans-acting regulatory factor 1, Transcription factor RFX1

Gene Symbol

RFX1

Additional RFX1 Products

Product Documents for RFX1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RFX1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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