RHAMM/CD168 Antibody (2F2C9) - BSA Free

Novus Biologicals | Catalog # NBP2-52437

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F2C9

Format

BSA Free
View all RHAMM/CD168 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human RHAMM/CD168 (AA: 306-497) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

84.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RHAMM/CD168 Antibody (2F2C9) - BSA Free

Western Blot: RHAMM/CD168 Antibody (2F2C9)BSA Free [NBP2-52437]

Western Blot: RHAMM/CD168 Antibody (2F2C9)BSA Free [NBP2-52437]

Western Blot: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis using CD168 mAb against HEK293 (1) and CD168 (AA: 306-497)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) - BSA Free [NBP2-52437]

Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) - BSA Free [NBP2-52437]

Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - An intracellular stain was performed on Jurkat cells with RHAMM/CD168 Antibody (2F2C9) NBP2-52437AF488 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Western Blot: RHAMM/CD168 Antibody (2F2C9)BSA Free [NBP2-52437]

Western Blot: RHAMM/CD168 Antibody (2F2C9)BSA Free [NBP2-52437]

Western Blot: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis using CD168 mAb against human CD168 (AA: 306-497) recombinant protein. (Expected MW is 48.3 kDa)
Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) - BSA Free [NBP2-52437]

Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) - BSA Free [NBP2-52437]

Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis of Hela cells using CD168 mouse mAb (green) and negative control (red).
Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) - BSA Free [NBP2-52437]

Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) - BSA Free [NBP2-52437]

Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - An intracellular stain was performed on A549 cells with RHAMM/CD168 Antibody (2F2C9) NBP2-52437PE and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) - BSA Free [NBP2-52437]

Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) - BSA Free [NBP2-52437]

Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - An intracellular stain was performed on Jurkat cells with RHAMM/CD168 Antibody (2F2C9) NBP2-52437PE and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
ELISA: RHAMM/CD168 Antibody (2F2C9) - BSA Free [NBP2-52437]

ELISA: RHAMM/CD168 Antibody (2F2C9) - BSA Free [NBP2-52437]

ELISA: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Applications for RHAMM/CD168 Antibody (2F2C9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RHAMM/CD168

The protein encoded by the CD168 gene is involved in cell motility. It is expressed in breast tissue and together with otherproteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer.Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. (provided byRefSeq)

Long Name

Receptor For Hyaluronan-Mediated Motility

Alternate Names

CD168, HMMR, IHABP

Gene Symbol

HMMR

Additional RHAMM/CD168 Products

Product Documents for RHAMM/CD168 Antibody (2F2C9) - BSA Free

Certificate of Analysis

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Product Specific Notices for RHAMM/CD168 Antibody (2F2C9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RHAMM/CD168 Antibody (2F2C9) - BSA Free

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Protocols

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FAQs

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