Rheb Antibody (GT39810) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13608

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Porcine (99%), Rhesus Macaque (100%), Sheep (99%), Xenopus (91%), Zebrafish (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT39810

Format

Azide and BSA Free
View all Rheb Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rheb. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Rheb Antibody (GT39810) - Azide and BSA Free

Western Blot: Rheb Antibody (GT39810) [NBP3-13608]

Western Blot: Rheb Antibody (GT39810) [NBP3-13608]

Western Blot: Rheb Antibody (GT39810) [NBP3-13608] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Rheb antibody [GT39810] (NBP3-13608) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Rheb Antibody (GT39810) [NBP3-13608]

Immunocytochemistry/ Immunofluorescence: Rheb Antibody (GT39810) [NBP3-13608]

Immunocytochemistry/Immunofluorescence: Rheb Antibody (GT39810) [NBP3-13608] - Rheb antibody [GT39810] detects Rheb protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rheb stained by Rheb antibody [GT39810] (NBP3-13608) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar= 10um.
Immunohistochemistry-Paraffin: Rheb Antibody (GT39810) [NBP3-13608]

Immunohistochemistry-Paraffin: Rheb Antibody (GT39810) [NBP3-13608]

Immunohistochemistry-Paraffin: Rheb Antibody (GT39810) [NBP3-13608] - Rheb antibody [GT39810] detects Rheb protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Rheb stained by Rheb antibody [GT39810] (NBP3-13608) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Rheb Antibody (GT39810) [NBP3-13608]

Immunohistochemistry-Paraffin: Rheb Antibody (GT39810) [NBP3-13608]

Immunohistochemistry-Paraffin: Rheb Antibody (GT39810) [NBP3-13608] - Rheb antibody [GT39810] detects Rheb protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Rheb stained by Rheb antibody [GT39810] (NBP3-13608) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Rheb Antibody (GT39810) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rheb

Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.

Long Name

Ras Homolog Enriched In Brain

Alternate Names

Rheb2

Gene Symbol

RHEB

Additional Rheb Products

Product Documents for Rheb Antibody (GT39810) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rheb Antibody (GT39810) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Interferons

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Protocols

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FAQs

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