RIPK1/RIP1 Antibody

Novus Biologicals | Catalog # NBP2-20166

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RIPK1/RIP1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RIPK1/RIP1. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RIPK1/RIP1 Antibody

RIPK1/RIP1 Antibody

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP2-20166] -

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP2-20166] - RIPK1/RIP1 antibody [C1C3] detects RIPK1/RIP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
RIPK1/RIP1 stained by RIPK1/RIP1 antibody [C1C3] (NBP2-20166) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RIPK1/RIP1 Antibody

Western Blot: RIPK1/RIP1 Antibody [NBP2-20166] -

Western Blot: RIPK1/RIP1 Antibody [NBP2-20166] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RIPK1/RIP1 antibody [C1C3] (NBP2-20166) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RIPK1/RIP1 Antibody

Western Blot: RIPK1/RIP1 Antibody [NBP2-20166] -

Western Blot: RIPK1/RIP1 Antibody [NBP2-20166] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RIPK1/RIP1 antibody [C1C3] (NBP2-20166) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for RIPK1/RIP1 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RIPK1/RIP1

Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.

Long Name

Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 1

Alternate Names

RIP, RIP1

Gene Symbol

RIPK1

UniProt

Q13546

Additional RIPK1/RIP1 Products

Product Documents for RIPK1/RIP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RIPK1/RIP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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