RKIP/PBP Antibody (003) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-90295

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 003 expressed in HEK293

Format

Azide and BSA Free
View all RKIP Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human RKIP/PBP (Accession#: NP_002558.1; Met1-Lys187).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for RKIP/PBP Antibody (003) - Azide and BSA Free

Western Blot: RKIP/PBP Antibody (003) [NBP2-90295]

Western Blot: RKIP/PBP Antibody (003) [NBP2-90295]

Western Blot: RKIP/PBP Antibody (003) [NBP2-90295] - Lane A: HeLa Whole Cell LysateLane B: A549 Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.)

Applications for RKIP/PBP Antibody (003) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RKIP

PBP, also known as RKIP (RAF kinase inhibitor protein), binds ATP, opioids and phosphatidylethanolamine. PBP is able to regulate Aurora kinase B expression and the spindle checkpoint through interaction with the RAF1/MEK/ERK cascade. PBP is able to bind to RAF1 and MEK and competitively disrupt the interaction between these proteins. PBP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. It increases the production of choline acetyltransferase but not acetylcholinesterase. PBP has been implicated as a suppressor of metastasis.

Long Name

Raf kinase inhibitory protein

Alternate Names

HCNPpp, Neuropolypeptide h3, PBP, PEBP-1, PEBP1

Gene Symbol

PEBP1

Additional RKIP Products

Product Documents for RKIP/PBP Antibody (003) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RKIP/PBP Antibody (003) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Serine Proteases and Regulators

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FAQs

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