RNA Helicase A Antibody

Novus Biologicals | Catalog # NBP1-28915

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RNA Helicase A Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence TEGRNALIHKSSVNC corresponding to internal region according to NP_001348.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

141 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNA Helicase A Antibody

Western Blot: RNA Helicase A Antibody [NBP1-28915]

Western Blot: RNA Helicase A Antibody [NBP1-28915]

Western Blot: RNA Helicase A Antibody [NBP1-28915] - (1ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for RNA Helicase A Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 140 kDa band observed in HeLa nuclear lysates (calculated MW of 141 kDa band according to NP_001348.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNA Helicase A

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein with RNA helicase activity. It may participate in melting of DNA:RNA hybrids, such as those that occur during transcription, and may play a role in X-linked gene expression. It contains 2 copies of a double-stranded RNA-binding domain, a DEXH core domain and an RGG box. The RNA-binding domains and RGG box influence and regulate RNA helicase activity. [provided by RefSeq]

Long Name

ATP-dependent RNA helicase A

Alternate Names

DDX9, DEAH box protein 9, DExH-box helicase 9, DHX9, EC 3.6.4.13, Leukophysin, LKP, NDH II, NDH2

Entrez Gene IDs

1660 (Human); 13211 (Mouse); 304859 (Rat)

Gene Symbol

DHX9

UniProt

Q08211 (Human)

Additional RNA Helicase A Products

Product Documents for RNA Helicase A Antibody

Certificate of Analysis

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Product Specific Notices for RNA Helicase A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RNA Helicase A Antibody

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