RNA polymerase beta Antibody (JE32-42)

Novus Biologicals | Catalog # NBP3-32909

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

E. coli

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE32-42 expressed in HEK293
View all RNA polymerase beta Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within E.coli RNA polymerase beta aa 980-1,342 / 1,342. (Uniprot: P0A8V2)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNA polymerase beta Antibody (JE32-42)

RNA polymerase beta Antibody (JE32-42)

Western Blot: RNA polymerase beta Antibody (JE32-42) [NBP3-32909]

Western Blot: RNA polymerase beta Antibody (JE32-42) [NBP3-32909] - Western blot analysis of RNA polymerase beta on different lysates with Rabbit anti-RNA polymerase beta antibody (NBP3-32909) at 1/2,000 dilution.

Lane 1: E.coli cell lysate
Lane 2: E.coli cell lysate (no heat)

Lysates/proteins at 10 ug/Lane.

Predicted band size: 150 kDa
Observed band size: 150 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32909) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for RNA polymerase beta Antibody (JE32-42)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNA polymerase beta

Gene Symbol

rpoB

Additional RNA polymerase beta Products

Product Documents for RNA polymerase beta Antibody (JE32-42)

Certificate of Analysis

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Download SDS

Product Specific Notices for RNA polymerase beta Antibody (JE32-42)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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