RNA Polymerase II/POLR2A [p Thr4] Antibody [Biotin]

Novus Biologicals | Catalog # NBP1-49546B

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Chicken, Drosophila, Yeast

Applications

Western Blot, Dot Blot

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

A synthetic peptide made to a phosphorylated Threonine (position 4) of the CTD heptad in the human RNA polymerase II protein [UniProt P24928]

Modification

p Thr4

Localization

Nucleus.

Specificity

This antibody is specific for the phosphorylated Threonine found in position 4 of the RNA polymerase II CTD heptad.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for RNA Polymerase II/POLR2A [p Thr4] Antibody [Biotin]

Application
Recommended Usage

Dot Blot

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: RNA Polymerase II/POLR2A

RNA polymerase II catalyzes DNA transcription into RNA, and is composed of 12 subunits. The largest of these subunits is B1 (RPB1, POLR2A), which contains the DNA binding domain and the enzymatic subunit. RPB1 contains multiple heptapeptide repeats in the CTD arranged in the sequence Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7 (YSPTSPS). Modifications of this sequence, such as phosphorylation, alter polymerase activity. Serines 2, 5, and 7 are known to become phosphorylated to lead the complex through the transcription cycle, as well as functions in polyadenylation, mRNA capping, and snRNA-processing.

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Gene Symbol

POLR2A

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A [p Thr4] Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RNA Polymerase II/POLR2A [p Thr4] Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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