RNA Polymerase II/POLR2A Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-55663

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RNA Polymerase II/POLR2A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNA Polymerase II/POLR2A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A Antibody [NBP2-55663]

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A Antibody [NBP2-55663]

Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A Antibody [NBP2-55663] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
RNA Polymerase II/POLR2A Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: RNA Polymerase II/POLR2A Antibody - BSA Free [NBP2-55663]

Chromatin Immunoprecipitation-exo-Seq: RNA Polymerase II/POLR2A Antibody - BSA Free [NBP2-55663]

ChIP-Exo-Seq composite graph for Anti-POLR2A (NBP2-55663) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for RNA Polymerase II/POLR2A Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNA Polymerase II/POLR2A

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Gene Symbol

POLR2A

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RNA Polymerase II/POLR2A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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