RNF20 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-2242

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RNF20 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Ring Finger 20 using the numbering given in entry NP_062538.5 (GeneID 56254).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNF20 Antibody - BSA Free

Western Blot: RNF20 Antibody [NB100-2242]

Western Blot: RNF20 Antibody [NB100-2242]

Western Blot: RNF20 Antibody [NB100-2242] - Detection of Human and Mouse RNF20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF20 antibody NB100-2242 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: RNF20 Antibody [NB100-2242]

Western Blot: RNF20 Antibody [NB100-2242]

Western Blot: RNF20 Antibody [NB100-2242] - WB analysis of RNF20 in HeLa whole cell lysate. Image submitted by a verified customer review.
Immunoprecipitation: RNF20 Antibody [NB100-2242]

Immunoprecipitation: RNF20 Antibody [NB100-2242]

Immunoprecipitation: RNF20 Antibody [NB100-2242] - Detection of human RNF20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF20 antibody NB100-2242 (lot NB100-2242-2) used for IP at 6 ug per reaction. RNF20 was also immunoprecipitated by a previous lot of this antibody (NB100-2242-1) and rabbit anti-RNF20 antibody NB100-2243. For blotting immunoprecipitated RNF20, NB100-2242 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
RNF20 Antibody - BSA Free

Western Blot: RNF20 Antibody - BSA Free [NB100-2242] -

Overexpression of H2BK120R efficiently reduced H2B monoubiquitination levels.a. HEK293T cells transfected with an RNF20-specific siRNA or a control siRNA and with a GFP-tagged H2BK120R mutant plasmid or an empty GFP plasmid were harvested after 48 h of transfection. Cell extracts were prepared and analyzed by western blot with the specified antibodies. b. HeLa cells were transfected with an RNF20-specific siRNA or a control siRNA and with a GFP-tagged H2BK120R mutant plasmid or an empty GFP plasmid. Cells were harvested after 48 h of transfection, and cell extracts were prepared. Western blot analysis was performed with the specified antibodies. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26177367), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
RNF20 Antibody - BSA Free

Western Blot: RNF20 Antibody - BSA Free [NB100-2242] -

Overexpression of H2BK120R efficiently reduced H2B monoubiquitination levels.a. HEK293T cells transfected with an RNF20-specific siRNA or a control siRNA and with a GFP-tagged H2BK120R mutant plasmid or an empty GFP plasmid were harvested after 48 h of transfection. Cell extracts were prepared and analyzed by western blot with the specified antibodies. b. HeLa cells were transfected with an RNF20-specific siRNA or a control siRNA and with a GFP-tagged H2BK120R mutant plasmid or an empty GFP plasmid. Cells were harvested after 48 h of transfection, and cell extracts were prepared. Western blot analysis was performed with the specified antibodies. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26177367), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RNF20 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Chip reactivity reported in (PMID: 21617703).

Reviewed Applications

Read 1 review rated 4 using NB100-2242 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RNF20

The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ring finger protein 20 (RNF20) is a member of the RING type E3 ubiquitin ligases. RNF20 forms a complex with RNF40 to carry out H2B monoubiquitination in association with hPAF and the UbcH6 ubiquitin E2-conjugating enzyme. H2B monoubiquitination by RNF40/RNF20 has been shown to be associated with increased H3 methylation and gene transcription. Alternate names for RNF20 include E3 ubiquitin-protein ligase BRE1A, BRE1-A, hBRE1, FLJ11189, FLJ20382, KIAA2779, MGC129667, and MGC129668.

Alternate Names

BRE1, BRE1 E3 ubiquitin ligase homolog, BRE1-A, BRE1AMGC129667, E3 ubiquitin-protein ligase BRE1A, EC 6.3.2, EC 6.3.2.-, FLJ11189, FLJ20382, hBRE1MGC129668, homolog of S. cerevisiae BRE1, KAIA2779, ring finger protein 20KIAA2779

Entrez Gene IDs

56254 (Human)

Gene Symbol

RNF20

UniProt

Q5VTR2 (Human)

Additional RNF20 Products

Product Documents for RNF20 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RNF20 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RNF20 Antibody - BSA Free

Customer Reviews for RNF20 Antibody - BSA Free (1)

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Customer Images

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  • Name: Mansi Arora
    Application: Western Blot
    Sample Tested: Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 05/16/2012
    RNF20 levels in HeLa cells after treatment with control (lane 1) or RNF20 (lane 2) siRNA
    RNF20 Antibody - BSA Free NB100-2242

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