ROCK1 Antibody (H5E4)

Name: ROCK1 Antibody (H5E4)
Brand: Novus Biologicals
SKU: NBP3-15350
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15350

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # H5E4 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human ROCK1 (Q13464). STSVASFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROCK1 Antibody (H5E4)

Western Blot: ROCK1 Antibody (H5E4) [NBP3-15350]

Western Blot: ROCK1 Antibody (H5E4) [NBP3-15350] - Western blot analysis of extracts of various cell lines, using ROCK1 Rabbit mAb (NBP3-15350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western Blot: ROCK1 Antibody (H5E4) [ROCK1] -

Western Blot: ROCK1 Antibody (H5E4) [ROCK1] - Western blot analysis of various lysates using ROCK1 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Applications for ROCK1 Antibody (H5E4)

Application

Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ROCK1

Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1; Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).

Long Name

Rho-associated, Coiled-Coil Containing Protein Kinase 1

Alternate Names

p160ROCK, ROK beta

Gene Symbol

ROCK1

Additional ROCK1 Products

Product Documents for ROCK1 Antibody (H5E4)

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Certificate of Analysis

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Product Specific Notices for ROCK1 Antibody (H5E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.