ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free

Novus Biologicals | Catalog # NBP2-24449

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Primate (96%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4G419

Format

BSA Free
View all ROR gamma/RORC/NR1F3 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 1-50 of human ROR gamma was used as the immunogen.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22110656).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free

Western Blot: ROR gamma/RORC/NR1F3 Antibody (4G419)BSA Free [NBP2-24449]

Western Blot: ROR gamma/RORC/NR1F3 Antibody (4G419)BSA Free [NBP2-24449]

Western Blot: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Analysis in (A) human thymus lysate, (B) transfected 293 cell lysate, and (C) untransfected 293 cell lysate. Dilution: 5 ug/ml.
Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free [NBP2-24449]

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free [NBP2-24449]

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Analysis using the FITC conjugate of NBP2-24449. Staining of ROR gamma in human lymphocytes using 10 ul (0.25 ug) of ROR gamma antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; red represents ROR gamma
Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free [NBP2-24449]

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free [NBP2-24449]

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Intracellular analysis of ROR gamma in human lymphocytes using 0.25 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody ; red represents ROR gamma antibody.
Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free [NBP2-24449]

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free [NBP2-24449]

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Intracellular flow cytometric analysis of ROR gamma in human monocytes using 0.25 ug of ROR gamma antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody (Novus, 20109); red represents ROR gamma antibody.
Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free [NBP2-24449]

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free [NBP2-24449]

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Analysis using the Allophycocyanin conjugate of NBP2-24449. Staining of ROR gamma in human lymphocytes using 10 ul (0.25 ug) of ROR gamma antibody. Shaded histogram represents cells without antibody; green represents isotype control antibodywas used for this test.

Applications for ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.25-0.5 ug/10^6 cells

Western Blot

5 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ROR gamma/RORC/NR1F3

ROR gamma/RORC/NR1F3, aka RAR Orphan Receptor gamma, is a Nuclear Receptor 1 Thyroid Hormone-Like receptor containing a short A-T rich sequence follow by a single core motif half-site 5'-AGGTCA-3'. RORC is a key regulator of thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. NR1F3 regulates intrinsic transcriptional functions. Unlike other major nuclear receptors, RORC binds as a monomer to hormone response elements and is documented in mouse thymus, adipose, bone, skeletal muscle, liver, kidney, and human skeletal muscle. An N-terminal isoform of ROR gamma, ROR gamma T, inhibits Fas ligand expression and cytokine secretion in immature thymocytes.

Long Name

Retinoic Acid-Related Orphan Receptor gamma

Alternate Names

NR1F3, RORC, RZRG, mouse ror gamma

Gene Symbol

RORC

Additional ROR gamma/RORC/NR1F3 Products

Product Documents for ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free

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FAQs for ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free

Showing  1 - 4 of 4 FAQs Showing All

  • Q: Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?

    A: Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.

  • Q: Since the protocol to isolate cells from mice seems to be quite long I would like to know if you have experience in staining with Foxp3 and Ror gamma and leaving cells overnight? Would you suggest leaving cells overnight in Fix/Perm or better after having completed the staining? And in which buffer would you leave them then?

    A: Where as FOXP3 and ROR gamma staining can be stopped after the fixation step, we suggest that researchers strictly follow the protocol without variation (even if it takes a long time), when trying for the 1st time. After you establish this for your system, then you can work on optimizing a varied protocol according to your needs and convenience. In this case, after the fix/perm step (Step 13 of NBP2-266911) wash the cells in 1X Staining buffer (2 times) and suspend in Staining buffer (500 ul) overnight. Then, in the morning proceed to Step 14 and continue the protocol.

  • Q: What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?

    A: A sequence from amino acids 1 - 50 of Human ROR gamma.

  • Q: What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?

    A: The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.

  • Q: Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?

    A: Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.

  • Q: Since the protocol to isolate cells from mice seems to be quite long I would like to know if you have experience in staining with Foxp3 and Ror gamma and leaving cells overnight? Would you suggest leaving cells overnight in Fix/Perm or better after having completed the staining? And in which buffer would you leave them then?

    A: Where as FOXP3 and ROR gamma staining can be stopped after the fixation step, we suggest that researchers strictly follow the protocol without variation (even if it takes a long time), when trying for the 1st time. After you establish this for your system, then you can work on optimizing a varied protocol according to your needs and convenience. In this case, after the fix/perm step (Step 13 of NBP2-266911) wash the cells in 1X Staining buffer (2 times) and suspend in Staining buffer (500 ul) overnight. Then, in the morning proceed to Step 14 and continue the protocol.

  • Q: What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?

    A: A sequence from amino acids 1 - 50 of Human ROR gamma.

  • Q: What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?

    A: The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.

  • Q: Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?

    A: Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.

  • Q: Since the protocol to isolate cells from mice seems to be quite long I would like to know if you have experience in staining with Foxp3 and Ror gamma and leaving cells overnight? Would you suggest leaving cells overnight in Fix/Perm or better after having completed the staining? And in which buffer would you leave them then?

    A: Where as FOXP3 and ROR gamma staining can be stopped after the fixation step, we suggest that researchers strictly follow the protocol without variation (even if it takes a long time), when trying for the 1st time. After you establish this for your system, then you can work on optimizing a varied protocol according to your needs and convenience. In this case, after the fix/perm step (Step 13 of NBP2-266911) wash the cells in 1X Staining buffer (2 times) and suspend in Staining buffer (500 ul) overnight. Then, in the morning proceed to Step 14 and continue the protocol.

  • Q: What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?

    A: A sequence from amino acids 1 - 50 of Human ROR gamma.

  • Q: What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?

    A: The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.

  • Q: Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?

    A: Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.

  • Q: Since the protocol to isolate cells from mice seems to be quite long I would like to know if you have experience in staining with Foxp3 and Ror gamma and leaving cells overnight? Would you suggest leaving cells overnight in Fix/Perm or better after having completed the staining? And in which buffer would you leave them then?

    A: Where as FOXP3 and ROR gamma staining can be stopped after the fixation step, we suggest that researchers strictly follow the protocol without variation (even if it takes a long time), when trying for the 1st time. After you establish this for your system, then you can work on optimizing a varied protocol according to your needs and convenience. In this case, after the fix/perm step (Step 13 of NBP2-266911) wash the cells in 1X Staining buffer (2 times) and suspend in Staining buffer (500 ul) overnight. Then, in the morning proceed to Step 14 and continue the protocol.

  • Q: What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?

    A: A sequence from amino acids 1 - 50 of Human ROR gamma.

  • Q: What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?

    A: The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.

Showing  1 - 4 of 4 FAQs Showing All
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