ROR1 Antibody (151A5) - Chimeric
Novus Biologicals | Catalog # NBP3-48407
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # 151A5
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Product Specifications
Immunogen
ROR1 recombinant protein expressed in mammalian cells (Accession# Q01973). The exact amino acid sequence is proprietary.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Description
This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).
Scientific Data Images for ROR1 Antibody (151A5) - Chimeric
Western Blot: ROR1 Antibody (151A5) - Chimeric [NBP3-48407]
Anti-ROR1 antibody (Catalog Number# NBP3-48407) at 1/1000 dilutionLane : 293T-ROR1, whole cell lysateSecondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilutionPredicted band size: 104 kDaObserved band size: 110 kDaFlow Cytometry: ROR1 Antibody (151A5) - Chimeric [NBP3-48407]
Flow cytometry analysis of antigen binding of anti-human ROR1 mAb(NBP3-48407). (A) NBP3-48407 does not bind to CHO-S cells that do not express ROR1. (B) A clear peak shift of NBP3-48407 was seen compared to the control when incubated with ROR1-expressing AGS cells, indicating strong binding of NBP3-48407 to ROR1. Antibodies were incubated at 5 ug/mL.Applications for ROR1 Antibody (151A5) - Chimeric
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified from cell culture supernatant
Reconstitution
Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.
Formulation
PBS, pH 7.4, 5-8% Trehalose
Preservative
Trehalose
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C in powder form. Store at -80C once reconstituted.
Calculators
Background: ROR1
Long Name
Receptor Tyrosine Kinase-like Orphan Receptor 1
Alternate Names
NTRKR1
Gene Symbol
ROR1
Additional ROR1 Products
Product Documents for ROR1 Antibody (151A5) - Chimeric
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ROR1 Antibody (151A5) - Chimeric
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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