RPA70 Antibody (4C4) - BSA Free

Novus Biologicals | Catalog # NBP2-37500

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4C4

Format

BSA Free
View all RPA70 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human RPA70 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPA70 Antibody (4C4) - BSA Free

Western Blot: RPA70 Antibody (4C4)BSA Free [NBP2-37500]

Western Blot: RPA70 Antibody (4C4)BSA Free [NBP2-37500]

Western Blot: RPA70 Antibody (4C4) [NBP2-37500] - Western blot analysis using RPA1 mouse mAb against HeLa (1), MCF-7 (2), K562(3), A431(4), and COS-7 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: RPA70 Antibody (4C4) - BSA Free [NBP2-37500]

Immunocytochemistry/ Immunofluorescence: RPA70 Antibody (4C4) - BSA Free [NBP2-37500]

Immunocytochemistry/Immunofluorescence: RPA70 Antibody (4C4) [NBP2-37500] - Immunofluorescence analysis of HeLa cells using RPA1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: RPA70 Antibody (4C4) - BSA Free [NBP2-37500]

Immunohistochemistry: RPA70 Antibody (4C4) - BSA Free [NBP2-37500]

Immunohistochemistry: RPA70 Antibody (4C4) [NBP2-37500] - Immunohistochemical analysis of paraffin-embedded prostate tissues using RPA1 mouse mAb with DAB staining.
Flow Cytometry: RPA70 Antibody (4C4) - BSA Free [NBP2-37500]

Flow Cytometry: RPA70 Antibody (4C4) - BSA Free [NBP2-37500]

Flow Cytometry: RPA70 Antibody (4C4) [NBP2-37500] - Flow cytometric analysis of Jurkat cells using RPA1 mouse mAb (green) and negative control (red).
Western Blot: RPA70 Antibody (4C4)BSA Free [NBP2-37500]

Western Blot: RPA70 Antibody (4C4)BSA Free [NBP2-37500]

Western Blot: RPA70 Antibody (4C4) [NBP2-37500] - Western blot analysis using RPA1 mAb against human RPA1 (AA: 308-513) recombinant protein. (Expected MW is 48.3 kDa)
Immunohistochemistry: RPA70 Antibody (4C4) - BSA Free [NBP2-37500]

Immunohistochemistry: RPA70 Antibody (4C4) - BSA Free [NBP2-37500]

Immunohistochemistry: RPA70 Antibody (4C4) [NBP2-37500] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using RPA1 mouse mAb with DAB staining.
ELISA: RPA70 Antibody (4C4) - BSA Free [NBP2-37500]

ELISA: RPA70 Antibody (4C4) - BSA Free [NBP2-37500]

ELISA: RPA70 Antibody (4C4) [NBP2-37500] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for RPA70 Antibody (4C4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPA70

RPA (Replication protein A) is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). RPA is a single strand binding protein that is involved in replication, homologous recombination and nucleotide excision and repair. It colocalises with RAD51 on synapsed axes in meiosis, facilitating RAD51 function. Replication protein A acts as an auxiliary protein for DNA polymerases alpha and delta.

Alternate Names

HSSB, MST075, MSTP075, REPA1RF-A protein 1, Replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, replication protein A1 (70kD), replication protein A1, 70kDa, RF-A, RP-A, RPA70RP-A p70, Single-stranded DNA-binding protein

Gene Symbol

RPA1

Additional RPA70 Products

Product Documents for RPA70 Antibody (4C4) - BSA Free

Certificate of Analysis

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Product Specific Notices for RPA70 Antibody (4C4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RPA70 Antibody (4C4) - BSA Free

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Protocols

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FAQs

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